Adenosine receptor A1 Antibodies

Adenosine receptor A1 (ADORA1)

Receptor for adenosine.

The activity of this receptor is mediated by G proteins that inhibit adenylyl cyclase.

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Gene Information

Human
Gene Name:	ADORA1
Uniprot:	P30542
Entrez:	134

Family

Belongs to:
G-protein coupled receptor 1 family

Alternative Names

Adenosine A1 R; adenosine A1 receptor; Adenosine A1R; ADORA 1; ADORA1; RDC7; RDC7adenosine receptor A1

Molecular Weight

Mass (kDA):
36.512 kDA

Genomic Context

Human
Location:	1q32.1
Sequence:	1; NC_000001.11 (203127705..203167405)

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Neoplasms
• Malignant Neoplasm Of Breast
• Carcinoma
• Malignant Neoplasms
• Scleral Diseases
• Pain
• Mammary Neoplasms
• Ischemia
• Myopia
• Neuralgia
• Chronic Pain
• Coronary Artery Disease

• Nervousness
• Nerve Damage
• Essential Hypertension
• Neoplasm Metastasis
• Metastatic Malignant Neoplasm To The Liver
• Hypoxia

Interacting Proteins

• ADORA2A
• ADRB1
• ADRB2
• TSHR

Pathways

• Pathogenesis
• Localization
• Vasodilation
• Smooth Muscle Contraction
• Reverse Transcription
• Cell Cycle
• Aging
• Muscle Contraction

• Penile Erection
• Cell Cycle Arrest

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ADORA1

Boster Bio - Best Uses Of The ADORA1 Marker

This article will focus on the ADORA1 marker's benefits. We will also discuss the requirements for submitting results to TSHR markers, and we will discuss the specific antibodies and sources offered by Boster Bio. We'll end up explaining why it's a great option for your research. Hopefully this article was helpful! Boster Bio can be used as a resource.

The ADORA1 Marker has many benefits

ADORA1 expression in hepatocellular tumors is elevated, which is the most prevalent malignancy. However, it is not clear if ADORA1 overexpression in HCC cells has any clinical significance. We have examined the levels ADORA1 expression in HCC cell lines and tissues. We also determined whether ADORA1 induced the growth of HCC tumours in vivo. We also tested the efficacy ADORA1 knockdown via flow cytometry as well as nuclei fragmentation.

ADORA1 is a gene expressed in synovial tissues of patients with rheumatoid arthritis. It is expressed more highly in synovia than in other joints, but it is not exclusively present. It is found all tissues, including cartilage. Chondrocytes, glial and neuronal cells. Benefits of using ADORA1 Marker

To perform the ADORA1 gene knockdown experiments, we used Lipofectamine 2000, an Invitrogen product, to transfect siRNA into cells. Each knockdown experiment used 200 pmoles of siRNA and two siRNA control siRNAs that were not-synonymous. Our results indicate that ADORA1 knockdown could help identify patients at high risk of developing the disease.

HepG2 cells are more likely to express ADORA1 than HepG2 ones. We also overexpressed ADORA1 in HepG2 cells with a pcDNA-3.1 vector, and HepG2-ADORA1 cell lines using G418 screening. Both cell lines showed that HepG2-ADORA1 cells grew faster than HepG2-control cells. In addition, we also observed enhanced invasion in transwell assays.

Requirements to submit results to the TSHR marker

The TSHR marker is an epigenetic gene that is expressed in many cancers, including the most common type of lung cancer, melanoma, and colorectal cancer. It is not expressed in all clinical tissues or in poorly differentiated DTC cell line. Its function has not yet been fully investigated. It is not yet clear if this marker plays any roles in the progression of HCC.

It is controlled by the thyroid-stimulatinghormone (TSH), a hormone that regulates cell cycles and regulates tumor growth. TSH receptor inhibition also prevents EMT, which can allow cancer cells spread to distant places. In studies, thyroid cancer cells that express TSHR are suppressed during metastasis and infiltrating other tumors, a sign that the TSHR marker may be a biomarker. Although its role is not yet clear, it could play an independent role in the clinic as a prognostic marker.

TSHR isoforms A and B are present in human lymphocytes. Both isoforms are bindable by the T cell receptors. It is possible that a soluble TSHR ioform is involved in central tolerance. Although this is unknown, these studies indicate that soluble isoforms are involved in central tolerance. These studies will continue to investigate the role of TSHR in the immune system.

The GD-associated Intron 1 polymorphisms can be found in the TSHR genes. The genes encode proteins which can be expressed as cell-anchored protein or soluble forms. GD is associated with approximately 30 kb. Each exon codes an amino acid. The numbers at either end of the gene diagram are the amino acids. The numbers at bottom represent the distances in km between the two regions. There are many primary associated SNPs with the TSHR marker. It also binds PLZF transcription factor.

Sources of antibodies

Studies have shown that ADORA1 expression is found in approximately 25% OSCCs. The exact mechanism of ADORA2B expression remains unknown. It may be linked to tumorigenesis. In this study, the expression of ADORA1 in oral cancers was compared to that in healthy oral tissues. Primary OSCC samples showed positive staining primarily in the cell cytoplasm. This resulted in patients with this form of cancer having higher ADORA2B IHC scores compared to those with normal oral tissue.

To detect the protein, rabbit anti-ADORA2B monoclonal antibody was used in conjunction with a mouse anti-HIF-1a antibody. After incubation at 30 % in hydrogen peroxide, membranes received Envision reagent. It developed color in the presence 3'-diminobenzidine.

ADORA2B, a key molecule in the immune response, is important. It is an integral part of the membrane protein that activates denylate cyclase if adenosine presence is present. Although its role in the immune response and inflammation is still poorly understood, ADORA2B is believed to play an important role in the progression of oral squamous cell carcinoma.

Boster Bio provides specific antibodies

A01101-1 antibody from the company is tested in ELISA or IF and reacts with both human ADORA1 DNA and rat ADORA1 genes. The company offers customized antibody production packages to scientists who need to test for discontinued or new antibodies. These packages typically cost less than $1,000, and can be used as a test for rare or noncommercial species. The price of custom antibody is comparable to regular antibodies.