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Product Info Summary
| SKU: | M00432 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal Antibody
SKU/Catalog Number
M00432
BM4557 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal Antibody catalog # M00432. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00432)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FIO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AGTR2
Reactive Species
M00432 is reactive to AGTR2 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
41.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00432 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human HepG2 whole cell, human SH-SY5Y whole cell, human Caco-2 whole cell, rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of AGTR2 using anti-AGTR2 antibody (M00432).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR2 antigen affinity purified monoclonal antibody (M00432) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGTR2 at approximately 45 kDa. The expected band size for AGTR2 is at 41 kDa.
Specific Publications For Anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal Antibody (M00432)
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6 Customer Q&As for Anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal Antibody
Question
Would anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 work for IP with uterus?
C. Johnson
Verified customer
Asked: 2020-03-26
Answer
According to the expression profile of uterus, AGTR2 is highly expressed in uterus. So, it is likely that anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 will work for IP with uterus.
Boster Scientific Support
Answered: 2020-03-26
Question
I see that the anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-16
Question
We are currently using anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
The anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody (M00432) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-14
Question
Is a blocking peptide available for product anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody (M00432)?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We do provide the blocking peptide for product anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody (M00432). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-11-01
Question
Is this M00432 anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody reactive to the isotypes of AGTR2?
G. Kulkarni
Verified customer
Asked: 2016-01-04
Answer
The immunogen of M00432 anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody is A synthesized peptide derived from human AGTR2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-04
Question
Would anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 work on horse WB with uterus?
R. Mitchell
Verified customer
Asked: 2013-05-13
Answer
Our lab technicians have not tested anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 on horse. You can run a BLAST between horse and the immunogen sequence of anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal antibody M00432 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-13
