Click image to see more details
Product Info Summary
| SKU: | A00267 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AID AICDA Antibody
SKU/Catalog Number
A00267
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AID AICDA Antibody (Catalog # A00267). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
AID antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AID AICDA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00267)
Host
Rabbit
Contents
AID Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID. The immunogen is located within the last 50 amino acids of AID.
Reactive Species
A00267 is reactive to AICDA in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
24.0 kDa
Background of AICDA
Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00267 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/mL in either the (A) absence or (B) presence of blocking peptide.
Click image to see more details
Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 μg/mL.
Click image to see more details
Immunofluorescence of AID in Ramos cells with AID antibody at 20 μg/mL.
Specific Publications For Anti-AID AICDA Antibody (A00267)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AID AICDA Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-AID AICDA Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
