Anti-AID AICDA Antibody

Product Name

Anti-AID AICDA Antibody

View all Aicda Antibodies

SKU/Catalog Number

A00267-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-AID AICDA Antibody catalog # A00267-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AID AICDA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00267-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human AID.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00267-1 is reactive to Aicda in Human, Mouse, Rat

Applications

A00267-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

24.031kDa

Background of Aicda

Histone H2A.X (phospho S139) antibody is ideal for western blotting and ELISA. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA is wrapped around histone-groups, consisting of the core histones H2A, H2B, H3 and H4. As a reaction on DNA Double-strand breaks (DSB) H2AX becomes phosphorylated on serine 139, called gamma-H2AX. ATM, ATR and PRKDCs, kinases of the PI3-family, are responsible for this phosphorylation. The modification can happen accidentally during replication fork collapse, exogenous genotoxic agents, may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes, in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. Mutagenesis experiments have shown that the modification is necessary for the proper formation of ionizing radiation induced foci in response to double strand breaks, but is not required for the recruitment of proteins to the site of DSBs. Gamma-H2AX is a sensitive target for looking at DSBs in cells. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. The role of the phosphorylated form of the histone in DNA repair is under. Anti-H2AX pS139 is ideal for researched interested in Histones, DNA Damage and Repair, and Epigenetics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC-P, 1:100-1:300

Validation Images & Assay Conditions

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Western Blot (WB) analysis of HeLa, HepG2 cells using AID Polyclonal antibody.

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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon Cancer, antibody was diluted at 1:100.

Gene/Protein Information For Aicda (Source: Uniprot.org, NCBI)

Alternative Names

activation-induced cytidine deaminase; AICDA; AID; AIDCytidine aminohydrolase; Arp2; CDA2; HIGM2; HIGM2EC 3.5.4.5; integrated into Burkitt's lymphoma cell line Ramos Aicda|A, Aid, Arp2|activation-induced cytidine deaminase|single-stranded DNA cytosine deaminase|activation induced deaminase|cytidine aminohydrolase

For more info on Aicda, check out the Aicda Infographic

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In this infographic, you will see the following information for Aicda: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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