Anti-Aim2 Antibody Picoband™

Product Name

Anti-Aim2 Antibody Picoband™

View all AIM2 Antibodies

SKU/Catalog Number

A00443-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aim2 Antibody Picoband™ catalog # A00443-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Aim2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00443-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Aim2 recombinant protein (Position: Q136-E354).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00443-1 is reactive to AIM2 in Mouse

Applications

A00443-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

38.954kDa

Background of AIM2

Interferon-inducible protein AIM2,also known asabsent in melanoma 2or simplyAIM2,is aproteinthat in humans is encoded by theAIM2gene. It is mapped to 1q22. AIM2 is a member of theIfi202/IFI16family. It plays a putative role in tumorigenic reversion and may control cell proliferation.Interferon-gammainduces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA(either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another proteinASC(or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains aCARD domain(caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation ofcaspase-1, an enzyme that processes proinflammatorycytokines(IL-1bandIL-18).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Flow Cytometry, 1-3 μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Aim2 using anti-Aim2 antibody (A00443-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aim2 antigen affinity purified polyclonal antibody (Catalog # A00443-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aim2 at approximately 39 kDa. The expected band size for Aim2 is at 39 kDa.

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Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-Aim2 antibody (A00443-1).
Overlay histogram showing RAW264.7 cells stained with A00443-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aim2 Antibody (A00443-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For AIM2 (Source: Uniprot.org, NCBI)

Alternative Names

absent in melanoma 2PYHIN4; AIM2; Gm1313; Ifi210; interferon-inducible protein AIM2; PYHIN4 AIM2 PYHIN4 absent in melanoma 2 interferon-inducible protein AIM2

For more info on AIM2, check out the AIM2 Infographic

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In this infographic, you will see the following information for AIM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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