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Product Info Summary
| SKU: | A07963 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-A-kinase anchor protein 11 AKAP11 Antibody
SKU/Catalog Number
A07963
Size
100ul
Form
Liquid
Description
Boster Bio Anti-A-kinase anchor protein 11 AKAP11 Antibody (Catalog# A07963). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-A-kinase anchor protein 11 AKAP11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07963)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AKAP11. Immunogen sequence location: 1761-1810
Reactive Species
A07963 is reactive to AKAP11 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
210.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07963 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using AKAP11 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP11 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-A-kinase anchor protein 11 AKAP11 Antibody (A07963)
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Customer Q&As
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6 Customer Q&As for Anti-A-kinase anchor protein 11 AKAP11 Antibody
Question
We need to test anti-AKAP11 antibody A07963 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-AKAP11 antibody A07963 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The products we sell, including anti-AKAP11 antibody A07963, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-22
Question
Would anti-AKAP11 antibody A07963 work for IF with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
According to the expression profile of cervix carcinoma erythroleukemia, AKAP11 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-AKAP11 antibody A07963 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-16
Question
Does A07963 anti-AKAP11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Anderson
Verified customer
Asked: 2018-11-14
Answer
As indicated on the product datasheet, A07963 anti-AKAP11 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-14
Question
We are currently using anti-AKAP11 antibody A07963 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
The anti-AKAP11 antibody (A07963) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-31
Question
I see that the anti-AKAP11 antibody A07963 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-15
Question
Is this A07963 anti-AKAP11 antibody reactive to the isotypes of AKAP11?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
The immunogen of A07963 anti-AKAP11 antibody is A peptide derived from human AKAP11. Immunogen sequence location: 1761-1810. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-29
