Anti-A-kinase anchor protein 13 AKAP13 Antibody

Product Name

Anti-A-kinase anchor protein 13 AKAP13 Antibody

View all AKAP13 Antibodies

SKU/Catalog Number

A03017

Size

100ul

Form

Liquid

Description

Boster Bio Anti-A-kinase anchor protein 13 AKAP13 Antibody (Catalog# A03017). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-A-kinase anchor protein 13 AKAP13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03017)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human AKAP13. Immunogen sequence location: 721-770

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03017 is reactive to AKAP13 in Human, Mouse, Rat

Applications

A03017 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

307.55kDa

Background of AKAP13

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP13 Antibody. The picture on the right is blocked with the synthesized peptide.

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Western blot analysis of lysates from Jurkat and A549 cells, using AKAP13 Antibody. The lane on the right is blocked with the synthesized peptide.

Gene/Protein Information For AKAP13 (Source: Uniprot.org, NCBI)

Alternative Names

A kinase (PRKA) anchor protein 13; AKAP-LbcAKAP-13; A-kinase anchor protein 13; A-kinase anchoring protein; ARHGEF13; Breast cancer nuclear receptor-binding auxiliary protein; BRXNon-oncogenic Rho GTPase-specific GTP exchange factor; c-lbc; FLJ11952; FLJ43341; Guanine nucleotide exchange factor Lbc; HA-3; HT31; Human thyroid-anchoring protein 31; LBCLBC oncogene; Lymphoid blast crisis oncogeneProtein kinase A-anchoring protein 13; p47; PROTO-LB; PROTO-LBC AKAP13 AKAP-13, AKAP-Lbc, ARHGEF13, BRX, HA-3, Ht31, LBC, PRKA13, PROTO-LB, PROTO-LBC, c-lbc, p47 A-kinase anchoring protein 13 A-kinase anchor protein 13|A kinase (PRKA) anchor protein 13|LBC oncogene|breast cancer nuclear receptor-binding auxiliary protein|guanine nucleotide exchange factor Lbc|human thyroid-anchoring protein 31|lymphoid blast crisis oncogene|non-oncogenic Rho GTPase-specific GTP exchange factor|protein kinase A-anchoring protein 13

For more info on AKAP13, check out the AKAP13 Infographic

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In this infographic, you will see the following information for AKAP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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