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Product Info Summary
| SKU: | A01820 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-AKR1C3 Antibody
SKU/Catalog Number
A01820
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AKR1C3 Antibody catalog # A01820. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKR1C3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01820)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human AKR1C3(NP_003730.4).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01820 is reactive to AKR1C3 in Human, Mouse
Calculated molecular weight
36.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01820 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of various lysates, using AKR1C3 antibody at 1:1620 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
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Immunofluorescence analysis of NIH/3T3 cells using AKR1C3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug AKR1C3 antibody . Western blot was performed from the immunoprecipitate using AKR1C3 antibody at a dilution of 1:1000.
Specific Publications For Anti-AKR1C3 Antibody (A01820)
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