Product Info Summary
SKU: | M07555 |
---|---|
Size: | 100ul |
Reactive Species: | Mouse |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Akt1 PXK Monoclonal Antibody
SKU/Catalog Number
M07555
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Akt1 PXK Monoclonal Antibody catalog # M07555. Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Akt1 PXK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07555)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
2D9-D2-H3
Isotype
IgG
Immunogen
Purified recombinant human AKT1 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07555 is reactive to PXK in Mouse
Applications
M07555 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
64.95kDa
Background of PXK
Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Akt1 PXK Monoclonal Antibody M07555
Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000)Click image to see more details
Figure 2. Western blotting validation for Anti-Akt1 PXK Monoclonal Antibody M07555
Western blot detection of AKT1 in CHO-K1 cell lysate (B) and CHO-K1 transfected by AKT1 (A) cell lysate using AKT1 mouse mAb (1:1000 diluted). Predicted band size: 60KDa. Observed band size: 60KDa.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PXK (Source: Uniprot.org, NCBI)
Gene Name
PXK
Full Name
PX domain-containing protein kinase-like protein
Weight
64.95kDa
Superfamily
protein kinase superfamily
Alternative Names
FLJ20335; K-ATPase long form; K-ATPase; modulator of Na; MONaKA; PX domain containing serine/threonine kinase; PX domain-containing protein kinase-like protein; PX ser/thr kinase v2; PX serine/threonine kinase PXK MONAKA, SLOB PX domain containing serine/threonine kinase like PX domain-containing protein kinase-like protein|PX ser/thr kinase v2|PX serine/threonine kinase|modulator of Na,K-ATPase long form
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PXK, check out the PXK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PXK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Akt1 PXK Monoclonal Antibody (M07555)
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No publications found for M07555
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