Anti-Alas1 Antibody Picoband™

ALAS1 antibody

Boster Bio Anti-Alas1 Antibody Picoband™ catalog # A10405-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A10405-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Alas1 Antibody Picoband™

View all ALAS1 Antibodies

SKU/Catalog Number

A10405-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alas1 Antibody Picoband™ catalog # A10405-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alas1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10405-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Alas1 recombinant protein (Position: M1-Q244).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10405-1 is reactive to ALAS1 in Human, Mouse, Rat

Applications

A10405-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

71 kDa

Calculated molecular weight

70.581kDa

Background of ALAS1

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALAS1 (Source: Uniprot.org, NCBI)

Gene Name

ALAS1

Full Name

5-aminolevulinate synthase, nonspecific, mitochondrial

Weight

70.581kDa

Superfamily

class-II pyridoxal-phosphate-dependent aminotransferase family

Alternative Names

5-aminolevulinic acid synthase 1; ALAS; ALAS3; ALASH; ALAS-H; aminolevulinate, delta-, synthase 1; Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial; Delta-aminolevulinate synthase 1; EC 2.3.1; EC 2.3.1.37; MIG4; migration-inducing protein 4 ALAS1 ALAS, ALAS-H, ALAS3, ALASH, MIG4 5-aminolevulinate synthase 1 5-aminolevulinate synthase, nonspecific, mitochondrial|5-aminolevulinic acid synthase 1|aminolevulinate, delta-, synthase 1|delta-ALA synthase 1|delta-aminolevulinate synthase 1|migration-inducing protein 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALAS1, check out the ALAS1 Infographic

ALAS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Size

Conjugation

Total: $370

SKU:A10405-1

In stock, 1 left.

Order within 31 hours and 9 minutes to receive by Thu Mar 21

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A10405-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

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