Product Info Summary
SKU: | PB10038 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-ALDH7A1 Antibody Picoband™
SKU/Catalog Number
PB10038
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ALDH7A1 Antibody Picoband™ catalog # PB10038. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALDH7A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10038)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH7A1, different from the related mouse sequence by eight amino acids, and from the related rat sequence by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10038 is reactive to ALDH7A1 in Human, Mouse, Rat
Applications
PB10038 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
58.487kDa
Background of ALDH7A1
Aldehyde dehydrogenase 7 family, member A1, also known as ALDH7A1 or antiquitin, is an enzyme that in humans is encoded by the ALDH7A1 gene. The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism andlipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH7A1 antigen affinity purified polyclonal antibody (Catalog # PB10038) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH7A1 at approximately 55 kDa. The expected band size for ALDH7A1 is at 55 kDa.
Click image to see more details
Figure 2. IHC analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).
ALDH7A1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).
ALDH7A1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).
ALDH7A1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of A431 cells using anti-ALDH7A1 antibody (PB10038).
Overlay histogram showing A431 cells stained with PB10038 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH7A1 Antibody (PB10038,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 6. IF analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).
ALDH7A1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ALDH7A1 (Source: Uniprot.org, NCBI)
Gene Name
ALDH7A1
Full Name
Alpha-aminoadipic semialdehyde dehydrogenase
Weight
58.487kDa
Superfamily
aldehyde dehydrogenase family
Alternative Names
aldehyde dehydrogenase 7 family, member A1; Aldehyde dehydrogenase family 7 member A1,26g turgor protein homolog; Alpha-AASA dehydrogenase; ATQ1Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; EC 1.2.1; EC 1.2.1.3; EC 1.2.1.31; EC 1.2.1.8; EPDalpha-aminoadipic semialdehyde dehydrogenase; FLJ11738; FLJ92814; P6c dehydrogenase; PDEantiquitin-1 ALDH7A1 ATQ1, EPD, PDE aldehyde dehydrogenase 7 family member A1 alpha-aminoadipic semialdehyde dehydrogenase|26g turgor protein homolog|P6c dehydrogenase|alpha-AASA dehydrogenase|antiquitin-1|betaine aldehyde dehydrogenase|delta1-piperideine-6-carboxylate dehydrogenase|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDH7A1, check out the ALDH7A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDH7A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALDH7A1 Antibody Picoband™ (PB10038)
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No publications found for PB10038
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Anti-ALDH7A1 Antibody Picoband™
Question
Is there a BSA free version of anti-ALDH7A1 antibody PB10038 available?
N. Moore
Verified customer
Asked: 2020-03-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALDH7A1 antibody PB10038 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-04
Question
Does anti-ALDH7A1 antibody PB10038 work for IHC-F with brain?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
According to the expression profile of brain, ALDH7A1 is highly expressed in brain. So, it is likely that anti-ALDH7A1 antibody PB10038 will work for IHC-F with brain.
Boster Scientific Support
Answered: 2019-01-08
Question
My question regarding product PB10038, anti-ALDH7A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Collins
Verified customer
Asked: 2018-12-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB10038 anti-ALDH7A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ALDH7A1 antibody PB10038. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-14
Question
I was wanting to use your anti-ALDH7A1 antibody for IHC-F for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
As indicated on the product datasheet, PB10038 anti-ALDH7A1 antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-06
Question
We are currently using anti-ALDH7A1 antibody PB10038 for mouse tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
The anti-ALDH7A1 antibody (PB10038) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-04
Question
Would anti-ALDH7A1 antibody PB10038 work on canine IHC-P with liver placenta?
W. Krishna
Verified customer
Asked: 2014-04-01
Answer
Our lab technicians have not tested anti-ALDH7A1 antibody PB10038 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ALDH7A1 antibody PB10038 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver placenta in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-01