Anti-GABA A Receptor alpha 1/GABRA1 Antibody
Rabbit IgG polyclonal antibody for Gamma-aminobutyric acid receptor subunit alpha-1(GABRA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-GABA A Receptor alpha 1/GABRA1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-GABA A Receptor alpha 1/GABRA1 Antibody
See all GABA-AR alpha 1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for GABA A R alpha 1/GABRA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GABA A R alpha 1/GABRA1 information: Molecular Weight: 51802 MW; Subcellular Localization: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.|
|Cite This Product||Anti-GABA A Receptor alpha 1/GABRA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1578)|
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|Anti-GABA A Receptor alpha 1/GABRA1 Antibody may replace the following items: sc 14005|sc 31403|sc 31404|sc 31405|sc 376282|sc 7348|sc 7349|sc 7352|sc 7356.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1(440-456aa ATYLNREPQLKAPTPHQ), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For GABRA1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2554|
|Species Of This Entry||Human|
|Protein Name||Gamma-aminobutyric acid receptor subunit alpha-1|
|Superfamily||ligand-gated ion channel (TC 1.A.9) family|
|Alternative Names||GABA-AR alpha 1|ECA4; EJM; EJM5GABA(A) receptor subunit alpha-1; GABA A R alpha 1; GABAAR alpha 1; GABAARa1; GABRA1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1|
|Post Tranlational Modifications||Phosphorylation; Methylation; Biotinylation|
|Subcellular Localization||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane.|
|Gene Location||5q34, on Chromasome 5, gene sequence: NC_000005.10 (161847191..161899971)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GABRA1 (Source: Uniprot.org, NCBI)
|Protein Function||Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand- gated chloride channel (By similarity). .|
|Research Areas||Neuronal Cell Markers; Neuroscience; Neurotransmission|
|Related Diseases||Epilepsy; Epilepsy, Generalized; Epilepsies, Myoclonic; Myoclonic Epilepsy, Juvenile; Nervousness; Absence Epilepsy; Schizophrenia; Febrile Convulsions; Anxiety Disorders; Depressive Disorder; Nervous System Disorder; Bipolar Disorder; Dysequilibrium Syndrome; Mood Disorders; Idiopathic Generalized Epilepsy; Neurodegenerative Disorders; Nerve Degeneration; Convulsion Neonatal|
|Related Pathways||Pathogenesis; Synaptic Transmission; Localization; Reflex; Righting Reflex; Translation; Methylation; Cell Death; Synapse Maturation; Brain Development; Cell Proliferation; Signal Transmission; Response To Hypoxia; Dna Methylation; Glycosylation; Startle Response; Drug Resistance; Locomotion; Innervation; Cell Adhesion|
|Background||GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97% homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.|
|Scientific References||PMID: 2465923 by Schofield P.R., et al. Sequence and expression of human GABAA receptor alpha 1 and beta 1 subunits.|
PMID: 2847710 by Garrett K.M., et al. Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.
Our Boster Quality Guarantee for Anti-GABA A Receptor alpha 1/GABRA1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
IHC(P): Rat Brain Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- GABRA1 antigen affinity purified polyclonal antibody (Catalog # PA1578) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRA1 at approximately 52KD. The expected band size for GABRA1 is at 52KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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