Product Info Summary
SKU: | A04688 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ALG3 Antibody Picoband™
SKU/Catalog Number
A04688
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ALG3 Antibody Picoband™ catalog # A04688. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ALG3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04688)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALG3 recombinant protein (Position: I61-H438).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04688 is reactive to ALG3 in Human, Mouse
Applications
A04688 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
50-55 kDa
Calculated molecular weight
50.126kDa
Background of ALG3
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase is an enzyme that, in humans, is encoded by the ALG3 gene. This gene encodes a member of the ALG3 family. The encoded protein catalyses the addition of the first dol-P-Man derived mannose in an alpha 1,3 linkage to Man5GlcNAc2-PP-Dol. Defects in this gene have been associated with congenital disorder of glycosylation type Id (CDG-Id) characterized by abnormal N-glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ALG3 using anti-ALG3 antibody (A04688).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: mouse ovary tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALG3 antigen affinity purified polyclonal antibody (Catalog # A04688) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALG3 at approximately 50-55 kDa. The expected band size for ALG3 is at 50 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of THP-1 cells using anti-ALG3 antibody (A04688).
Overlay histogram showing THP-1 cells stained with A04688 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALG3 Antibody (A04688, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of RT4 cells using anti-ALG3 antibody (A04688).
Overlay histogram showing RT4 cells stained with A04688 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALG3 Antibody (A04688, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ALG3 (Source: Uniprot.org, NCBI)
Gene Name
ALG3
Full Name
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Weight
50.126kDa
Superfamily
glycosyltransferase 58 family
Alternative Names
alpha-1,3-mannosyltransferase); asparagine-linked glycosylation 3 homolog (S. cerevisiae; asparagine-linked glycosylation 3 homolog (yeast; asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S.cerevisiae); Asparagine-linked glycosylation protein 3 homolog; carbohydrate deficient glycoprotein syndrome type IV; CDGS4D16Ertd36e; Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase; dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase; dol-P-Man dependent alpha(1-3)-mannosyltransferase; Dol-P-Man-dependent alpha(1-3)-mannosyltransferase; EC 2.4.1.130; ALG3 CDG1D, CDGS4, CDGS6, D16Ertd36e, NOT56L, Not56, not ALG3 alpha-1,3- mannosyltransferase dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase|Not56-like protein|asparagine-linked glycosylation 3 homolog (S. cerevisiae, alpha-1,3-mannosyltransferase)|asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase)|asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog|asparagine-linked glycosylation protein 3 homolog|carbohydrate deficient glycoprotein syndrome type IV|dol-P-Man-dependent alpha(1-3)-mannosyltransferase|dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase|dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALG3, check out the ALG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALG3 Antibody Picoband™ (A04688)
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