Click image to see more details
Product Info Summary
| SKU: | M01751 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ALIX Rabbit Monoclonal Antibody
SKU/Catalog Number
M01751
BM5496 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ALIX Rabbit Monoclonal Antibody catalog # M01751. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALIX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01751)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEBO-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ALIX
Reactive Species
M01751 is reactive to PDCD6IP in Human, Mouse, Rat
Observed Molecular Weight
96 kDa
Calculated molecular weight
96.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01751 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:100
Positive Control
WB: human K562 whole cell, human Raji whole cell, human Daudi whole cell, rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ALIX using anti-ALIX antibody (M01751).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified monoclonal antibody (Catalog # M01751) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALIX at approximately 96 kDa. The expected band size for ALIX is at 96 kDa.
Specific Publications For Anti-ALIX Rabbit Monoclonal Antibody (M01751)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ALIX Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ALIX Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-ALIX Rabbit Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01751?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of ALIX expression in K562 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We have been able to see staining in human osteosarcoma. Are there any suggestions? Is anti-ALIX Monoclonal antibody supposed to stain osteosarcoma positively?
D. Patel
Verified customer
Asked: 2019-09-03
Answer
Based on literature osteosarcoma does express PDCD6IP. Based on Uniprot.org, PDCD6IP is expressed in corpus callosum, lymph testis, osteosarcoma, placenta, brain, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have PDCD6IP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Lymph, and Testis, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-03
Question
Our lab were content with the WB result of your anti-ALIX Monoclonal antibody. However we have observed positive staining in brain cytosol using this antibody. Is that expected? Could you tell me where is PDCD6IP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
From literature, brain does express PDCD6IP. Generally PDCD6IP expresses in cytoplasm, cytosol. Regarding which tissues have PDCD6IP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Lymph, and Testis, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-07-13
Question
We are currently using anti-ALIX Monoclonal antibody M01751 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The anti-ALIX Monoclonal antibody (M01751) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-01
