Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody

ALK/CD246 antibody

Boster Bio Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (Catalog # M00301). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00301
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, WB

Product Name

Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody

View all ALK/CD246 Antibodies

SKU/Catalog Number

M00301

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (Catalog # M00301). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00301)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ALK/1503

Isotype

IgG1, kappa

Immunogen

Recombinant human ALK protein fragment (aa1360-1460)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00301 is reactive to ALK in Human

Applications

M00301 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

176.442kDa

Background of ALK/CD246

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This monoclonal antibody also reacts with blood vessels that serves as an internal positive control.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ALK (Source: Uniprot.org, NCBI)

Gene Name

ALK

Full Name

ALK tyrosine kinase receptor

Weight

176.442kDa

Superfamily

protein kinase superfamily

Alternative Names

ALK tyrosine kinase receptor; ALK; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246 antigen; CD246; EC 2.7.10.1; Ki-1; NBLST3; Tcrz ALK CD246, NBLST3 ALK receptor tyrosine kinase ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma receptor tyrosine kinase|mutant anaplastic lymphoma kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALK, check out the ALK Infographic

ALK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody

Question

What is the host species for M00301?

Verified customer

Asked: 2022-06-07

Answer

The host species for the Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (M00301) is Mouse.

Boster Scientific Support

Answered: 2022-06-07

Question

My colleagues were content with the WB result of your anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody. However we have been able to see positive staining in sperm cell membrane using this antibody. Is that expected? Could you tell me where is ALK supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

From what I have seen in literature, sperm does express ALK. Generally ALK expresses in cell membrane. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621

Boster Scientific Support

Answered: 2019-09-27

Question

We are currently using anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody M00301 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

The anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody (M00301) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-04

Question

We have seen staining in human brain. Any tips? Is anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2018-02-13

Answer

From what I have seen in literature brain does express ALK. From what I have seen in Uniprot.org, ALK is expressed in sperm, brain, among other tissues. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621

Boster Scientific Support

Answered: 2018-02-13

Question

I am looking for using your anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody for positive regulation of dendrite development studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

I appreciate your inquiry. This M00301 anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-12-28

Size

Total: $468

SKU:M00301

Backordered.

Lead time for this item is typically 6-8 days

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M00301
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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