Product Info Summary
SKU: | M00301 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody
SKU/Catalog Number
M00301
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (Catalog # M00301). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00301)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: ALK/1503
Isotype
IgG1, kappa
Immunogen
Recombinant human ALK protein fragment (aa1360-1460)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00301 is reactive to ALK in Human
Applications
M00301 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
176.442kDa
Background of ALK/CD246
The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This monoclonal antibody also reacts with blood vessels that serves as an internal positive control.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Analysis (A) Recombinant Protein (B) HepG2 Cell lysate Using Anti-ALK-1 Monoclonal Antibody (ALK/1503).
Protein Target Info & Infographic
Gene/Protein Information For ALK (Source: Uniprot.org, NCBI)
Gene Name
ALK
Full Name
ALK tyrosine kinase receptor
Weight
176.442kDa
Superfamily
protein kinase superfamily
Alternative Names
ALK tyrosine kinase receptor; ALK; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246 antigen; CD246; EC 2.7.10.1; Ki-1; NBLST3; Tcrz ALK CD246, NBLST3 ALK receptor tyrosine kinase ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma receptor tyrosine kinase|mutant anaplastic lymphoma kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALK, check out the ALK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (M00301)
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No publications found for M00301
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody
Question
What is the host species for M00301?
Verified customer
Asked: 2022-06-07
Answer
The host species for the Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal Antibody (M00301) is Mouse.
Boster Scientific Support
Answered: 2022-06-07
Question
My colleagues were content with the WB result of your anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody. However we have been able to see positive staining in sperm cell membrane using this antibody. Is that expected? Could you tell me where is ALK supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
From what I have seen in literature, sperm does express ALK. Generally ALK expresses in cell membrane. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody M00301 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody (M00301) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
We have seen staining in human brain. Any tips? Is anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
From what I have seen in literature brain does express ALK. From what I have seen in Uniprot.org, ALK is expressed in sperm, brain, among other tissues. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621
Boster Scientific Support
Answered: 2018-02-13
Question
I am looking for using your anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody for positive regulation of dendrite development studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
I appreciate your inquiry. This M00301 anti-ALK (Anaplastic Lymphoma Kinase) / CD246 Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-12-28