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Product Info Summary
| SKU: | A12775-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Alkaline ceramidase 1 ACER1 Antibody
SKU/Catalog Number
A12775-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Alkaline ceramidase 1 ACER1 Antibody catalog # A12775-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alkaline ceramidase 1 ACER1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12775-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ORAOV1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12775-1 is reactive to ACER1 in Human, Mouse, Rat
Applications
A12775-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
31.095kDa
Background of ASAH3
Belongs to the ORAOV1 family.
Huang X., Proc. Natl. Acad. Sci. U.S.A. 99:11369-11374(2002).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of ACER1 using Anti-Alkaline ceramidase 1 ACER1 Antibody (A12775-1).
Immunohistochemistry (IHC) analyzes of ACER1 pAb bin paraffin-embedded human liver carcinoma tissue at 1:50
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Figure 1. Western blotting validation for Anti-Alkaline ceramidase 1 ACER1 Antibody A12775-1
Western blot (WB) analysis of ACER1 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ACER1 (Source: Uniprot.org, NCBI)
Gene Name
ACER1
Full Name
Alkaline ceramidase 1
Weight
31.095kDa
Superfamily
alkaline ceramidase family
Alternative Names
Acylsphingosine deacylase 3; Alkaline CDase 1; alkaline ceramidase 1; AlkCDase 1; ALKCDase1; ASAH3; EC 3.5.1.23; MGC138327; MGC138329; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3; N-acylsphingosine amidohydrolase 3 ACER1 ALKCDase1, ASAH3 alkaline ceramidase 1 alkaline ceramidase 1|CTB-180A7.3|N-acylsphingosine amidohydrolase (alkaline ceramidase) 3|N-acylsphingosine amidohydrolase 3|acylsphingosine deacylase 3|alkCDase 1|alkaline CDase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACER1, check out the ACER1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACER1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alkaline ceramidase 1 ACER1 Antibody (A12775-1)
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No publications found for A12775-1
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