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- Table of Contents
ALPL antibody (M01008-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .
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Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody
ALPL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
ALPL antibody was positively detected by WB in:
human HepG2 whole cell, human Jurkat whole cell, human MCF-7 whole cell, human K562 whole cell, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALPL antibody was positively detected by IHC in:
human liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ALPL antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody
View all ALPL Antibodies
View all ALPL Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DBF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Alkaline Phosphatase
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated Molecular Weight
57.3 kDa
Cite This Product
Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01008-1)
Protocols
Western Blot protocol for ALPL antibody
Immunohistochemistry (IHC) protocol for ALPL antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ALPL antibody
Publications
Application:
Species:
Specnuezhenide Alleviates Senile Osteoporosis by Activating TGR5/FXR Signaling in Bone Marrow Mesenchymal Stem Cells and RANKL-Induced Osteoclasts
Published: 2025-03-05
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 40066080
Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling
Published: 2024-05-21
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38776853
Preparation of an injectable and photocurable carboxymethyl cellulose/hydroxyapatite composite and its application in cranial regeneration
Published: 2024-02-27
Journal: CARBOHYDRATE POLYMERS
Application: IHC
Impact Factor: 10.70
Species: Rat
PMID: 38494238
Nanosized Silk–Magnesium Complexes for Promotion of Angiogenic and Osteogenic Activities
Published: 2024-02-15
Journal: ACS Applied Materials & Interfaces
Application: IF
Impact Factor: 8.30
Species: Rat
PMID: 38359078
Expression of the HIF-1α/VEGF pathway is upregulated to protect alveolar bone density reduction in nasal-obstructed rats.
Published: 2024-01-02
Journal: HISTOLOGY AND HISTOPATHOLOGY
Application: WB
Impact Factor: 2.50
Species: Rat
PMID: 38235568
Explorating the mechanism of Epimedii folium-Rhizoma drynariae herbal pair promoted bone defects healing through network pharmacology and experimental studies
Published: 2023-10-24
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Rat
PMID: 37879510
Connexin43 overexpression promotes bone regeneration by osteogenesis and angiogenesis in rat glucocorticoid-induced osteonecrosis of the femoral head
Published: 2023-02-18
Journal: DEVELOPMENTAL BIOLOGY
Application: WB,IF,IHC
Impact Factor: 2.70
Species: Rat
PMID: 36805498
Injectable silk/hydroxyapatite nanocomposite hydrogels with vascularization capacity for bone regeneration
Published: 2020-03-05
Journal: JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
Application: IF
Impact Factor: 6.16
Species: Rat
Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons
Published: 2013-10-26
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: IF
Impact Factor: 1.29
Species: Human
PMID: 24163158
Review
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Product Promise
We promise Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01008-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026