Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband™

Product Name

Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband™

View all alpha-Fetoprotein/AFP Antibodies

SKU/Catalog Number

A00522

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband™ catalog # A00522. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00522)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Alpha 1 Fetoprotein recombinant protein (Position: K19-L254).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00522 is reactive to AFP in Mouse, Rat

Applications

A00522 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

68.678kDa

Background of alpha-Fetoprotein/AFP

RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Alpha 1 Fetoprotein using anti-Alpha 1 Fetoprotein antibody (A00522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Fetoprotein antigen affinity purified polyclonal antibody (Catalog # A00522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Fetoprotein at approximately 69KD. The expected band size for Alpha 1 Fetoprotein is at 69KD.

Click image to see more details

Figure 2. Western blot analysis of Alpha 1 Fetoprotein using anti-Alpha 1 Fetoprotein antibody (A00522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Fetoprotein antigen affinity purified polyclonal antibody (Catalog # A00522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Fetoprotein at approximately 69KD. The expected band size for Alpha 1 Fetoprotein is at 69KD.

Gene/Protein Information For AFP (Source: Uniprot.org, NCBI)

Alternative Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin

For more info on AFP, check out the AFP Infographic

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In this infographic, you will see the following information for AFP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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