Anti-Alpha 1B-Glycoprotein/A1BG Antibody Picoband™

Product Name

Anti-Alpha 1B-Glycoprotein/A1BG Antibody Picoband™

View all alpha 1B-Glycoprotein Antibodies

SKU/Catalog Number

A07289-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha 1B-Glycoprotein/A1BG Antibody Picoband™ catalog # A07289-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Alpha 1B-Glycoprotein/A1BG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07289-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human IDI1 recombinant protein (Position: M1-M227). Human IDI1 shares 86.8% amino acid (aa) sequence identity with both mouse and rat IDI1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A07289-2 is reactive to A1BG in Human, Mouse, Rat

Applications

A07289-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

50-75 kDa

Calculated molecular weight

54.254kDa

Background of alpha 1B-Glycoprotein

Alpha-1-B glycoprotein is a 54.3 kDa protein in humans that is encoded by the A1BG gene. The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Alpha 1B-Glycoprotein/A1BG using anti-Alpha 1B-Glycoprotein/A1BG antibody (A07289-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 3: rat plasma lysates,
Lane 4: mouse plasma lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG antigen affinity purified polyclonal antibody (Catalog # A07289-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1B-Glycoprotein/A1BG at approximately 50-75 kDa. The expected band size for Alpha 1B-Glycoprotein/A1BG is at 54 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Alpha 1B-Glycoprotein/A1BG antibody (A07289-2).
Overlay histogram showing HepG2 cells stained with A07289-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha 1B-Glycoprotein/A1BG Antibody (A07289-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For A1BG (Source: Uniprot.org, NCBI)

Alternative Names

A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; GAB A1BG A1B, ABG, GAB, HYST2477 alpha-1-B glycoprotein alpha-1B-glycoprotein|HEL-S-163pA|epididymis secretory sperm binding protein Li 163pA

For more info on A1BG, check out the A1BG Infographic

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In this infographic, you will see the following information for A1BG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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