Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, 35A47)

Product Name

Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, 35A47)

View all ACTN2 Antibodies

SKU/Catalog Number

M03673-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, 35A47) catalog # M03673-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, 35A47) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03673-2)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

EA-53

Isotype

Mouse IgG1

Immunogen

Rabbit skeletal alpha-actinin.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M03673-2 is reactive to ACTN2 in Human, Mouse, Rabbit, Rat

Background of ACTN2

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03673-2 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, rabbit, -
Western blot, 0.5-1μg/ml, Human, mouse, rat, rabbit

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information ACTN2

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