Product Info Summary
SKU: | MA1104 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rabbit, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)
View all Alpha Actinin 2 Antibodies
SKU/Catalog Number
MA1104
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) catalog # MA1104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1104)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
EA-53
Isotype
Mouse IgG1
Immunogen
Rabbit skeletal alpha-actinin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1104 is reactive to ACTN2 in Human, Mouse, Rabbit, Rat
Applications
MA1104 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
103 kDa
Calculated molecular weight
103.854kDa
Background of Alpha Actinin 2
Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, rabbit, -
Western blot, 0.5-1μg/ml, Human, mouse, rat, rabbit
Validation Images & Assay Conditions
Click image to see more details
Figure 3. IHC analysis of Alpha-Actinin using anti-Alpha-Actinin antibody (MA1104).
Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti-Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Alpha-Actinin using anti-Alpha-Actinin antibody (MA1104).
Alpha-Actinin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti-Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of Alpha-Actinin using anti-Alpha-Actinin antibody (MA1104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat cardiac muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse cardiac muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Alpha-Actinin antigen affinity purified monoclonal antibody (Catalog # MA1104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Alpha-Actinin at approximately 103KD. The expected band size for Alpha-Actinin is at 103KD.
Protein Target Info & Infographic
Gene/Protein Information For ACTN2 (Source: Uniprot.org, NCBI)
Gene Name
ACTN2
Full Name
Alpha-actinin-2
Weight
103.854kDa
Superfamily
alpha-actinin family
Alternative Names
Actinin alpha 2; actinin, alpha 2; Actinin-a2; ACTN2; alpha Actinin 2; alpha-Actinin 2; Alpha-actinin skeletal muscle isoform 2; Alpha-Actinin Skeletal Muscle; Alpha-Actinin-2; CMD1AA; F-actin cross-linking protein ACTN2 CMD1AA, CMH23, MPD6, MYOCOZ actinin alpha 2 alpha-actinin-2|F-actin cross-linking protein|alpha-actinin skeletal muscle|truncated actinin alpha 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTN2, check out the ACTN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) (MA1104)
Hello CJ!
MA1104 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hu H,Xue J,Dong R,Zhao Y,Song C,Zhao H,Hescheler J,Zhang Y,Liang H.STAT3 Phosphorylation Mediating DMSO‘s Function on Fetal Cardiomyocyte Proliferation with Developmental Changes.Int Heart J.2019 Mar 20;60(2):392-399.doi:10.1536/ihj.18-206.Epub 2019 Feb 8.PMID:30745528.
Species: Mouse
MA1104 usage in article: APP:IHC, SAMPLE:CARDIOMYOCYTES, DILUTION:1:100
Shen X,Zhang J,Zhang X,Wang Y,Hu Y,Guo J.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.Cell Mol Neurobiol.2019 Mar;39(2):241-254.doi:10.1007/s10571-018-0644-z.Epub 2018 Dec 15.PMID:30554401.
Species: Human
MA1104 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:1:300
Resveratrol alleviates hypoxia/reoxygenation injury-induced mitochondrial oxidative stress in cardiomyocytes Tao Li,1,* Linlin Chen,1,2,* Yiyan Yu,1 Binbin Yang,1 Pengyun Li,1 and Xiao-Qiu Tan1 Mol Med Rep. 2019 Apr; 19(4): 2774–2780. Published online
STAT3 Phosphorylation Mediating DMSO's Function on Fetal Cardiomyocyte Proliferation with Developmental Changes Haitao Hu, et al. Int Heart J. 2019 Mar 20;60(2):392-399. doi: 10.1536/ihj.18-206. Epub 2019 Feb 8.
Similar to Spironolactone, Oxymatrine Is Protective in Aldosterone-Induced Cardiomyocyte Injury via Inhibition of Calpain and Apoptosis-Inducing Factor Signaling
Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes
Xiaodan Chang, Baohong Zhang, Li Lihua, Zhichun Feng Am J Transl Res. 2016 Nov 15;8(11):4694-4704. eCollection 2016. T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism.
Li H, Tang M, Liang H, Li Y, Wang J, Song Y, Zheng Y, Xi J, Zhang J, Hescheler J, Zhu M. Cell Physiol Biochem. 2013;32(1):53-63. Doi: 10.1159/000350124. Epub 2013 Jul 4. Coculture Of Embryonic Ventricular Myocytes And Mouse Embryonic Stem Cell Enh...
Xiao T, Zhang Y, Wang Y, Xu Y, Yu Z, Shen X. Food Chem Toxicol. 2013 Mar;53:364-70. Doi: 10.1016/J.Fct.2012.12.022. Epub 2012 Dec 22. Activation Of An Apoptotic Signal Transduction Pathway Involved In The Upregulation Of Calpain And Apoptosis-Indu...
Chen Sf, Rao Ly, Wei Tz, Xu Mg, Dong Zl. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):796-800. Doi: 10.1007/S11596-014-1355-4. Epub 2014 Dec 6. Expression Patterns Of Sarcomeric ??-Actin, ??-Actinin And Ucp2 In The Myocardium Of Kunming M...
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Customer Q&As
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1 Customer Q&As for Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)
Question
We are currently using anti-Alpha-Actinin antibody (Monoclonal, EA-53) MA1104 for rabbit tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rabbit, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-Alpha-Actinin antibody (Monoclonal, EA-53) (MA1104) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08