Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)
Mouse IgG monoclonal antibody for alpha-Actinin, actinin, alpha 2 (ACTN2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat;rabbit. No cross reactivity with other proteins. Cited in 7 publication(s).
Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) Info At A Glance
|Reactivity:||Human, Mouse, Rabbit, Rat|
|Product Name||Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for ACTN2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ACTN2 information: Molecular Weight: 103834 MW; Subcellular Localization: ACTN2: Cytoplasm, myofibril, sarcomere, Z line|Actn2: Cytoplasm, myofibril, sarcomere, Z line; Tissue Specificity: ACTN2: Expressed in both skeletal and cardiac muscle.|
|Cite This Product||Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1104)|
|Similar Products From
|Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) may replace the following items: sc 130928|sc 15335|sc 166524|sc 17829|sc 7453|sc 7454 R.|
|Reactivity/Species||Human, Mouse, Rabbit, Rat|
|Predicted Reactivity||Monkey, Rabbit
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||Rabbit skeletal alpha-actinin.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ACTN2 (Source: Uniprot.org, NCBI)
|Molecular Weight||103834 MW|
|Subcellular Localization||ACTN2: Cytoplasm, myofibril, sarcomere, Z line|Actn2: Cytoplasm, myofibril, sarcomere, Z line|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ACTN2 (Source: Uniprot.org, NCBI)
|Protein Function||ACTN2: F-actin cross-linking protein which is thought to anchoractin to a variety of intracellular structures. This is a bundlingprotein.|
|Tissue Specificity||ACTN2: Expressed in both skeletal and cardiac muscle.|
|Background||Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.|
Our Boster Quality Guarantee for Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, rabbit, -
Western blot, 0.5-1μg/ml, Human, mouse, rat, rabbit
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Alpha-Actinin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat cardiac muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse cardiac muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Actinin antigen affinity purified monoclonal antibody (Catalog # MA1104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Alpha-Actinin at approximately 103KD. The expected band size for Alpha-Actinin is at 103KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 7
|Li M, Shang Yx. Bmc Pulm Med. 2012 Dec 17;12:79. Doi: 10.1186/1471-2466-12-79. Inhaled Corticosteroids Inhibit Substance P Receptor Expression In Asthmatic Rat Airway Smooth Muscle Cells.||PubMed ID 23244548|
|Chen Sf, Rao Ly, Wei Tz, Xu Mg, Dong Zl. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):796-800. Doi: 10.1007/S11596-014-1355-4. Epub 2014 Dec 6. Expression Patterns Of Sarcomeric ??-Actin, ??-Actinin And Ucp2 In The Myocardium Of Kunming M...||PubMed ID 25480572|
|Xiao T, Zhang Y, Wang Y, Xu Y, Yu Z, Shen X. Food Chem Toxicol. 2013 Mar;53:364-70. Doi: 10.1016/J.Fct.2012.12.022. Epub 2012 Dec 22. Activation Of An Apoptotic Signal Transduction Pathway Involved In The Upregulation Of Calpain And Apoptosis-Indu...||PubMed ID 23266505|
|Li H, Tang M, Liang H, Li Y, Wang J, Song Y, Zheng Y, Xi J, Zhang J, Hescheler J, Zhu M. Cell Physiol Biochem. 2013;32(1):53-63. Doi: 10.1159/000350124. Epub 2013 Jul 4. Coculture Of Embryonic Ventricular Myocytes And Mouse Embryonic Stem Cell Enh...||PubMed ID 23867886|
|Xiaodan Chang, Baohong Zhang, Li Lihua, Zhichun Feng Am J Transl Res. 2016 Nov 15;8(11):4694-4704. eCollection 2016. T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism.||PubMed ID 27904672|
|Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes||PubMed ID 25120772|
|Similar to Spironolactone, Oxymatrine Is Protective in Aldosterone-Induced Cardiomyocyte Injury via Inhibition of Calpain and Apoptosis-Inducing Factor Signaling||PubMed ID 24551180|
Order Product (MA1104)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.