Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)

Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) catalog # MA1104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat. Cited in 11 publication(s).

Product Info Summary

SKU: MA1104
Size: 100μg/vial
Reactive Species: Human, Mouse, Rabbit, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)

See all Alpha Actinin 2 products

SKU/Catalog Number

MA1104

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) catalog # MA1104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1104)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

EA-53

Isotype

Mouse IgG1

Immunogen

Rabbit skeletal alpha-actinin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1104 is reactive to ACTN2 in Human, Mouse, Rabbit, Rat

Applications

MA1104 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, rabbit, -
Western blot, 0.5-1μg/ml, Human, mouse, rat, rabbit

Validation Images & Assay Conditions

Gene/Protein Information For ACTN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P35609

Gene ID

88

Gene Name

ACTN2

Full Name

Alpha-actinin-2

Weight

103.854kDa

Superfamily

alpha-actinin family

Alternative Names

Actinin alpha 2; actinin, alpha 2; Actinin-a2; ACTN2; alpha Actinin 2; alpha-Actinin 2; Alpha-actinin skeletal muscle isoform 2; Alpha-Actinin Skeletal Muscle; Alpha-Actinin-2; CMD1AA; F-actin cross-linking protein ACTN2 CMD1AA, CMH23, MPD6, MYOCOZ actinin alpha 2 alpha-actinin-2|F-actin cross-linking protein|alpha-actinin skeletal muscle|truncated actinin alpha 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACTN2, check out the ACTN2 Infographic

ACTN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACTN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1104 has been cited in 11 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hu H,Xue J,Dong R,Zhao Y,Song C,Zhao H,Hescheler J,Zhang Y,Liang H.STAT3 Phosphorylation Mediating DMSO‘s Function on Fetal Cardiomyocyte Proliferation with Developmental Changes.Int Heart J.2019 Mar 20;60(2):392-399.doi:10.1536/ihj.18-206.Epub 2019 Feb 8.PMID:30745528.
Species: Mouse
MA1104 usage in article: APP:IHC, SAMPLE:CARDIOMYOCYTES, DILUTION:1:100

Shen X,Zhang J,Zhang X,Wang Y,Hu Y,Guo J.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.Cell Mol Neurobiol.2019 Mar;39(2):241-254.doi:10.1007/s10571-018-0644-z.Epub 2018 Dec 15.PMID:30554401.
Species: Human
MA1104 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:1:300

Resveratrol alleviates hypoxia/reoxygenation injury-induced mitochondrial oxidative stress in cardiomyocytes Tao Li,1,* Linlin Chen,1,2,* Yiyan Yu,1 Binbin Yang,1 Pengyun Li,1 and Xiao-Qiu Tan1 Mol Med Rep. 2019 Apr; 19(4): 2774–2780. Published online

STAT3 Phosphorylation Mediating DMSO's Function on Fetal Cardiomyocyte Proliferation with Developmental Changes Haitao Hu, et al. Int Heart J. 2019 Mar 20;60(2):392-399. doi: 10.1536/ihj.18-206. Epub 2019 Feb 8.

Similar to Spironolactone, Oxymatrine Is Protective in Aldosterone-Induced Cardiomyocyte Injury via Inhibition of Calpain and Apoptosis-Inducing Factor Signaling

Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes

Xiaodan Chang, Baohong Zhang, Li Lihua, Zhichun Feng Am J Transl Res. 2016 Nov 15;8(11):4694-4704. eCollection 2016. T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism.

Li H, Tang M, Liang H, Li Y, Wang J, Song Y, Zheng Y, Xi J, Zhang J, Hescheler J, Zhu M. Cell Physiol Biochem. 2013;32(1):53-63. Doi: 10.1159/000350124. Epub 2013 Jul 4. Coculture Of Embryonic Ventricular Myocytes And Mouse Embryonic Stem Cell Enh...

Xiao T, Zhang Y, Wang Y, Xu Y, Yu Z, Shen X. Food Chem Toxicol. 2013 Mar;53:364-70. Doi: 10.1016/J.Fct.2012.12.022. Epub 2012 Dec 22. Activation Of An Apoptotic Signal Transduction Pathway Involved In The Upregulation Of Calpain And Apoptosis-Indu...

Chen Sf, Rao Ly, Wei Tz, Xu Mg, Dong Zl. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):796-800. Doi: 10.1007/S11596-014-1355-4. Epub 2014 Dec 6. Expression Patterns Of Sarcomeric ??-Actin, ??-Actinin And Ucp2 In The Myocardium Of Kunming M...

Li M, Shang Yx. Bmc Pulm Med. 2012 Dec 17;12:79. Doi: 10.1186/1471-2466-12-79. Inhaled Corticosteroids Inhibit Substance P Receptor Expression In Asthmatic Rat Airway Smooth Muscle Cells.

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1 Customer Q&As for Anti-Alpha-Actinin ACTN2 Antibody (Monoclonal, EA-53)

Question

We are currently using anti-Alpha-Actinin antibody (Monoclonal, EA-53) MA1104 for rabbit tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rabbit, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

The anti-Alpha-Actinin antibody (Monoclonal, EA-53) (MA1104) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-08

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