radio
SKU MA1104
Size 100μg/vial
Reactivity Human, Mouse, Rabbit, Rat
Clonality Monoclonal
Clone Number EA-53
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)
SKU/Catalog Number MA1104
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for alpha-Actinin, actinin, alpha 2 (ACTN2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Alpha-Actinin Antibody (Monoclonal, EA-53) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1104)
Host Mouse
Immunogen Rabbit skeletal alpha-actinin.
Reactivity Human, Mouse, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, rabbit, -
Western blot, 0.5-1μg/ml, Human, mouse, rat, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1104 1_1 WB anti alpha actinin antibody monoclonal ea 53.jpg

Figure 1. Western blot analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat cardiac muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse cardiac muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Actinin antigen affinity purified monoclonal antibody (Catalog # MA1104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Alpha-Actinin at approximately 103KD. The expected band size for Alpha-Actinin is at 103KD.

/antibody/ma1104 2 IHC anti alpha actinin antibody monoclonal ea 53.jpg

Figure 2. IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104).
Alpha-Actinin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/antibody/ma1104 3 IHC anti alpha actinin antibody monoclonal ea 53.jpg

Figure 3. IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104).
Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D3ZCV0
Gene Name Actn2
Protein Name actinin, alpha 2
Tissue Specificity ACTN2: Expressed in both skeletal and cardiac muscle.
Alternative Names Protein Actn2 ;RCG30552 ;Actn2 ;rCG_30552 ;
Subcellular Localization ACTN2: Cytoplasm, myofibril, sarcomere, Z line|Actn2: Cytoplasm, myofibril, sarcomere, Z line
Molecular Weight 103834 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function ACTN2: F-actin cross-linking protein which is thought to anchoractin to a variety of intracellular structures. This is a bundlingprotein.
Background Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.

Order Product (MA1104)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.