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Product Info Summary
| SKU: | M03756 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-alpha Internexin INA Monoclonal Antibody
SKU/Catalog Number
M03756
BM5524 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-alpha Internexin INA Monoclonal Antibody catalog # M03756. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha Internexin INA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03756)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AECG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human alpha Internexin Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Reactive Species
M03756 is reactive to INA in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
55.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03756 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:20
Positive Control
WB: SHSY5Y cell
Validation Images & Assay Conditions
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Western blot analysis of alpha Internexin expression in SHSY5Y cell lysate.
Specific Publications For Anti-alpha Internexin INA Monoclonal Antibody (M03756)
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3 Customer Q&As for Anti-alpha Internexin INA Monoclonal Antibody
Question
I have a question about product M03756, anti-alpha Internexin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03756 anti-alpha Internexin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-30
Question
Does anti-alpha Internexin Monoclonal antibody M03756 work on zebrafish IP with brain?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
Our lab technicians have not validated anti-alpha Internexin Monoclonal antibody M03756 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-alpha Internexin Monoclonal antibody M03756 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-02
Question
I was wanting to use your anti-alpha Internexin Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
G. Li
Verified customer
Asked: 2013-11-22
Answer
It shows on the product datasheet, M03756 anti-alpha Internexin Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-22
