Anti-alpha-protein Kinase 1 ALPK1 Antibody

Alpk1 antibody

Boster Bio Anti-alpha-protein Kinase 1 ALPK1 Antibody catalog # A08171. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A08171
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

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Product Name

Anti-alpha-protein Kinase 1 ALPK1 Antibody

View all Alpk1 Antibodies

SKU/Catalog Number

A08171

Size

100μl

Form

Liquid

Description

Boster Bio Anti-alpha-protein Kinase 1 ALPK1 Antibody catalog # A08171. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-alpha-protein Kinase 1 ALPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08171)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human ALPK1 at amino acid range 11-60

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08171 is reactive to Alpk1 in Human, Mouse

Applications

A08171 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

136.134kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Validation Images & Assay Conditions

Gene/Protein Information For Alpk1 (Source: Uniprot.org, NCBI)

Gene Name

Alpk1

Full Name

Alpha-protein kinase 1

Weight

136.134kDa

Superfamily

protein kinase superfamily

Alternative Names

alpha-kinase 1; alpha-protein kinase 1; Chromosome 4 kinase; EC 2.7.11.-; KIAA15278430410J10Rik; Lak; LAKFLJ22670; lymphocyte alpha-kinase; Lymphocyte alpha-protein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Alpk1, check out the Alpk1 Infographic

Alpk1 infographic

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In this infographic, you will see the following information for Alpk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-alpha-protein Kinase 1 ALPK1 Antibody

Question

Can you please provide the percentage identity between the immunogen sequence and the corresponding sequence of the mouse ALPK1? Also I would apprecite it, if you could provide the range of amino acids in which the immunogen sequence is located? mouse ALPK1 has multiple isoforms and I would like to know which isoforms can get detected by your antibody.

Verified customer

Asked: 2022-08-05

Answer

Immunogen: The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60.

Boster Scientific Support

Answered: 2022-08-05

Question

Has A08171 been tested on mouse tissue or the reactive species is just based on identical percentage between the sequence of the immunogen and mouse ALPK1?

Verified customer

Asked: 2022-07-05

Answer

Unfortunately, we haven't tested the Anti-Alpha-Protein Kinase 1 ALPK1 Antibody (A08171) on mouse tissue. It was customer reported and we don’t have the resulting data.

Boster Scientific Support

Answered: 2022-07-07

Question

Will anti- alpha-protein Kinase 1 antibody A08171 work for WB with spleen?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

According to the expression profile of spleen, ALPK1 is highly expressed in spleen. So, it is likely that anti- alpha-protein Kinase 1 antibody A08171 will work for WB with spleen.

Boster Scientific Support

Answered: 2019-11-28

Question

We are currently using anti- alpha-protein Kinase 1 antibody A08171 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?

A. Jackson

Verified customer

Asked: 2019-09-06

Answer

The anti- alpha-protein Kinase 1 antibody (A08171) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-06

Question

Is a blocking peptide available for product anti- alpha-protein Kinase 1 antibody (A08171)?

B. Wu

Verified customer

Asked: 2017-06-29

Answer

We do provide the blocking peptide for product anti- alpha-protein Kinase 1 antibody (A08171). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-29

Question

Would A08171 anti- alpha-protein Kinase 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Williams

Verified customer

Asked: 2015-05-04

Answer

You can see on the product datasheet, A08171 anti- alpha-protein Kinase 1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-05-04

Question

See attached the WB image, lot number and protocol we used for spleen using anti- alpha-protein Kinase 1 antibody A08171. Please let me know if you require anything else.

K. Yang

Verified customer

Asked: 2014-09-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-09-22

Size

Total: $399

SKU:A08171

Backordered.

Lead time for this item is typically 2-4 weeks

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A08171
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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