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Product Info Summary
| SKU: | A08171 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-alpha-protein Kinase 1 ALPK1 Antibody
SKU/Catalog Number
A08171
Size
100μl
Form
Liquid
Description
Boster Bio Anti-alpha-protein Kinase 1 ALPK1 Antibody catalog # A08171. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha-protein Kinase 1 ALPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08171)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60
Reactive Species
A08171 is reactive to ALPK1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
138.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08171 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using α-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from COLO and HepG2 cells, using ALPK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-alpha-protein Kinase 1 ALPK1 Antibody (A08171)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-alpha-protein Kinase 1 ALPK1 Antibody
Question
Can you please provide the percentage identity between the immunogen sequence and the corresponding sequence of the mouse ALPK1? Also I would apprecite it, if you could provide the range of amino acids in which the immunogen sequence is located? mouse ALPK1 has multiple isoforms and I would like to know which isoforms can get detected by your antibody.
Verified customer
Asked: 2022-08-05
Answer
Immunogen： The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60.
Boster Scientific Support
Answered: 2022-08-05
Question
Has A08171 been tested on mouse tissue or the reactive species is just based on identical percentage between the sequence of the immunogen and mouse ALPK1?
Verified customer
Asked: 2022-07-05
Answer
Unfortunately, we haven't tested the Anti-Alpha-Protein Kinase 1 ALPK1 Antibody (A08171) on mouse tissue. It was customer reported and we don’t have the resulting data.
Boster Scientific Support
Answered: 2022-07-07
Question
Will anti- alpha-protein Kinase 1 antibody A08171 work for WB with spleen?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
According to the expression profile of spleen, ALPK1 is highly expressed in spleen. So, it is likely that anti- alpha-protein Kinase 1 antibody A08171 will work for WB with spleen.
Boster Scientific Support
Answered: 2019-11-28
Question
We are currently using anti- alpha-protein Kinase 1 antibody A08171 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
A. Jackson
Verified customer
Asked: 2019-09-06
Answer
The anti- alpha-protein Kinase 1 antibody (A08171) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-06
Question
Is a blocking peptide available for product anti- alpha-protein Kinase 1 antibody (A08171)?
B. Wu
Verified customer
Asked: 2017-06-29
Answer
We do provide the blocking peptide for product anti- alpha-protein Kinase 1 antibody (A08171). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-06-29
Question
Would A08171 anti- alpha-protein Kinase 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Williams
Verified customer
Asked: 2015-05-04
Answer
You can see on the product datasheet, A08171 anti- alpha-protein Kinase 1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-04
Question
See attached the WB image, lot number and protocol we used for spleen using anti- alpha-protein Kinase 1 antibody A08171. Please let me know if you require anything else.
K. Yang
Verified customer
Asked: 2014-09-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-22
