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Product Info Summary
| SKU: | M04756 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody
SKU/Catalog Number
M04756
BM5157 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody catalog # M04756. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04756)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABGE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human alpha Sarcoglycan
Reactive Species
M04756 is reactive to SGCA in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
42.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04756 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:40
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of alpha Sarcoglycan expression in Human skeletal muscle lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Specific Publications For Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody (M04756)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody
Question
Would anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756 work for IP with heart ventricle?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
According to the expression profile of heart ventricle, SGCA is highly expressed in heart ventricle. So, it is likely that anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756 will work for IP with heart ventricle.
Boster Scientific Support
Answered: 2019-11-01
Question
Is this M04756 anti-alpha Sarcoglycan Rabbit Monoclonal antibody reactive to the isotypes of SGCA?
O. Rodriguez
Verified customer
Asked: 2019-10-09
Answer
The immunogen of M04756 anti-alpha Sarcoglycan Rabbit Monoclonal antibody is A synthesized peptide derived from human alpha Sarcoglycan. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-09
Question
My lab would like to test anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756 on rat heart ventricle for research purposes, then I may be interested in using anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The products we sell, including anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-09
Question
Please see the WB image, lot number and protocol we used for heart ventricle using anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756. Please let me know if you require anything else.
G. Collins
Verified customer
Asked: 2018-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-22
Question
Is a blocking peptide available for product anti-alpha Sarcoglycan Rabbit Monoclonal antibody (M04756)?
N. Krishna
Verified customer
Asked: 2018-05-18
Answer
We do provide the blocking peptide for product anti-alpha Sarcoglycan Rabbit Monoclonal antibody (M04756). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-18
Question
I see that the anti-alpha Sarcoglycan Rabbit Monoclonal antibody M04756 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-12-15
Question
I was wanting to use your anti-alpha Sarcoglycan Rabbit Monoclonal antibody for IP for rat heart ventricle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat heart ventricle identification?
J. Banerjee
Verified customer
Asked: 2014-07-14
Answer
You can see on the product datasheet, M04756 anti-alpha Sarcoglycan Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart ventricle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-14
