Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57)

Product Name

Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57)

View all ACTA2 Antibodies

SKU/Catalog Number

M01072-7
BM0002 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57) catalog # MA1106. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-7)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

35A57

Isotype

Mouse IgG2a

Immunogen

N-terminal synthetic decapeptide of alpha-smooth muscle actin.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M01072-7 is reactive to ACTA2 in Human, Mouse, Rat

Calculated molecular weight

42.0 kDa

Background of ACTA2

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01072-7 is guaranteed for IF, IHC, IHC-F, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, mouse, rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, mouse, rat
Immunohistochemistry (Frozen Section), 2-5μg/ml, Human, mouse, rat,
Immunofluorescence, 5 μg/ml, Human, Rat

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information ACTA2

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