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- Table of Contents
ACTA2 antibody (M01072-7) performs well in IF, IHC, IHC-F, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of Alpha-Smooth Muscle Actin using anti-Alpha-Smooth Muscle Actin antibody (M01072-7).
Alpha-Smooth Muscle Actin was detected in a paraffin-embedded section of normal human uterine smooth muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Alpha-Smooth Muscle Actin Antibody (M01072-7) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Alpha-Smooth Muscle Actin was detected in a paraffin-embedded section of normal human uterine smooth muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Alpha-Smooth Muscle Actin Antibody (M01072-7) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57)
ACTA2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IF, IHC, IHC-F, WB |
Tested Applications
ACTA2 antibody was positively detected by IF in:
normal human uterine smooth muscle tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, mouse, rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, mouse, rat
Immunohistochemistry (Frozen Section)
2-5μg/ml
Human, mouse, rat,
Immunofluorescence
5 μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
Human
Recommend
Recommend
Mouse
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57)
View all ACTA2 Antibodies
View all ACTA2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
35A57
Isotype
Mouse IgG2a
Immunogen
N-terminal synthetic decapeptide of alpha-smooth muscle actin.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Calculated Molecular Weight
42.0 kDa
Background of ACTA2
Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
Cite This Product
Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-7)
Protocols
Immunofluorescence (IF) protocol for ACTA2 antibody
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Product Promise
We promise Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 35A57) performs excellently in relevant applications for IF, IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M01072-7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026