Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

alpha-Smooth Muscle Actin antibody

Boster Bio Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody catalog # M01072-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M01072-3
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

View all alpha-Smooth Muscle Actin Antibodies

SKU/Catalog Number

M01072-3

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody catalog # M01072-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-3)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CAD-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human alpha smooth muscle Actin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01072-3 is reactive to ACTA2 in Human, Mouse, Rat

Applications

M01072-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

42.009kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:100-1:500
ICC/IF 1:100-1:500
FC 1:30

Validation Images & Assay Conditions

Gene/Protein Information For ACTA2 (Source: Uniprot.org, NCBI)

Gene Name

ACTA2

Full Name

Actin, aortic smooth muscle

Weight

42.009kDa

Superfamily

actin family

Alternative Names

1a4 sma; 1a4 smooth muscle actin; AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha sma 1a4; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; sma 1a4; SMA; smooth muscle actin ACTA2 ACTSA actin alpha 2, smooth muscle actin, aortic smooth muscle|actin, alpha 2, smooth muscle, aorta|alpha-cardiac actin|cell growth-inhibiting gene 46 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTA2, check out the ACTA2 Infographic

ACTA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01072-3 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Supriono S,Kalim H,Permatasari N,Susianti H.Moringa oleifera Inhibits Liver Fibrosis Progression by Inhibition of α-Smooth Muscle Actin, Tissue Inhibitors of Metalloproteinases-1, and Collagen-1 in Rat Model Liver Fibrosis.Open Access Maced J Med Sci [Int
Species: Rat
M01072-3 usage in article: APP:IF, SAMPLE:LIVER TISSUE, DILUTION:1:500

Fang L,Wang KK,Zhang PF,Li T,Xiao ZL,Yang M,Yu ZX.Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF-BB.J Cell Mol Med.2020 Jan;24(2) :1917-1933.doi:10.1111/jcmm.14888.Epub 2020 Jan 1.PMI
Species: Rat
M01072-3 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA

Shen J,Wei W,Wang X,Yang J,Lu L,Lv X,Xue X.Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation.Evid Based Complement Alternat Med.2020 Aug 22;2020:72
Species: Rat
M01072-3 usage in article: APP:IHC, SAMPLE:VSMCS, DILUTION:1:1000

Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M01072-3 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000

Wang R, Wu G, Dai T, Lang Y, Chi Z, Yang S, Dong D. Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation. Exp Ther Med. 2021 Jan;21(1):66. doi: 10.3892/etm.2020.9498. Epub 2020 Nov 23. PMID: 33365
Species: Rat
M01072-3 usage in article: APP:IF, SAMPLE:KIDNEY TISSUE, DILUTION:1:70

Liao J, Wu Q, Qian C, Zhao N, Zhao Z, Lu K, Zhang S, Dong Q, Chen L, Li Q, Du Y. TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats. JCI Insight. 2020 Oct 29:137528. doi: 10.1172/jci.insight.137528. Epub ahead of print. PMID: 33119
Species: Rat
M01072-3 usage in article: APP:IHC, SAMPLA:ATRIA TISSUE, DILUTION:1:50

Yang J, Li J, Tan R, He X, Lin X, Zhong X, Fan J, Wang L. Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation. Phytother Res. 2020 Oct 28. doi:10.1002/ptr.6 919. Epub ahead of print. PMID: 33118280.
Species: Mouse
M01072-3 usage in article: APP:WB, SAMPLE:MOUSE KIDNEY TISSUES, DILUTION:1:1000

Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers

Extracorporeal shock wave therapy with low-energy flux density inhibits hypertrophic scar formation in an animal model

The Role and Mechanism of ?-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells

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4 Customer Q&As for Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

Question

Do you have a BSA free version of anti-alpha smooth muscle Actin Rabbit Monoclonal antibody M01072-3 available?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-alpha smooth muscle Actin Rabbit Monoclonal antibody M01072-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-27

Question

Does anti-alpha smooth muscle Actin Rabbit Monoclonal antibody M01072-3 work for WB with skeletal muscle?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

According to the expression profile of skeletal muscle, ACTA2 is highly expressed in skeletal muscle. So, it is likely that anti-alpha smooth muscle Actin Rabbit Monoclonal antibody M01072-3 will work for WB with skeletal muscle.

Boster Scientific Support

Answered: 2019-12-05

Question

I have a question about product M01072-3, anti-alpha smooth muscle Actin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01072-3 anti-alpha smooth muscle Actin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-alpha smooth muscle Actin Rabbit Monoclonal antibody M01072-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-07

Size

Total: $370

SKU:M01072-3

Backordered.

Lead time for this item is typically 3-4 weeks

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M01072-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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