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ACTA2 antibody (M01072-3) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 30 Publications

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m01072 3 alpha sma primary antibodies if testing 1

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IF analysis of alpha-SMAusing anti-alpha-SMA antibody (M01072-3).
alpha-SMA was detected in a paraffin-embedded section of human tosil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated at 1:50 rabbit anti-alpha-SMA Antibody (M01072-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

ACTA2 antibody

30 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

ACTA2 antibody was positively detected by WB in:

human A431 whole cell, human A549 whole cell, human Hela whole cellrat C6 whole cellmouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTA2 antibody was positively detected by IHC in:

human breast carcinoma tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ACTA2 antibody was positively detected by IF in:

human tosil tissue, rat brain vessel tissu

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Tested

Recommend

Rat

Recommend

Tested

Recommend

Mouse

Recommend

Product Information

Product Name

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
View all ACTA2 Antibodies

Form

Liquid

Uniprot

P62736

Gene ID

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

CAD-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human alpha smooth muscle Actin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

42 kDa

Calculated Molecular Weight

42.0 kDa

Cite This Product

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-3)

Protocols

Western Blot protocol for ACTA2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ACTA2 antibody

Review Protocol

Immunofluorescence (IF) protocol for ACTA2 antibody

Review Protocol

Publications

The m6A reader IGF2BP2 promotes hepatic fibrosis by stabilizing AEBP1 mRNA and regulating GRB2-mediated hepatic stellate cell activation

Published: 2026-07-27

Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

Application: IHC,IF,WB

Impact Factor: 5.00

Species: Mouse,Human

PMID: 42508775

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Free Secondary Antibody

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

ACTA2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

The m6A reader IGF2BP2 promotes hepatic fibrosis by stabilizing AEBP1 mRNA and regulating GRB2-mediated hepatic stellate cell activation

LncRNA ANRIL inhibits TGF-β1-induced proliferation, migration, and fibrosis of Tenon’s capsule fibroblasts from glaucoma patients by reducing IL-22-RA1 stability and expression

SPI1 promotes endothelial cell ferroptosis and intracranial aneurysm progression via EP300-mediated FOSL2 acetylation

NUDT21 Downregulation Promotes Liver Fibrosis by Activating the PIM2-Mediated CEBPB/SLC2A1 Glycolytic Axis in Hepatic Stellate Cells

Histologic and immunohistochemical changes in amputated and scarring lizard limb of Scincella tsinlingensis

Methylprednisolone attenuates tendon adhesion via modulating the eIF3a-TGF-β1 Axis in tenocytes and CCS-ROS-NLRP3 Axis in macrophages

ULK1 activates NCOA4‑mediated ferritinophagy via the Beclin1/VPS34 complex in cardiomyocyte hypertrophy

Icariin sensitizes glucocorticoid therapy in doxorubicin-induced fibrotic nephrotic syndrome via the HIF-1α/NF-κB/HDAC2 Axis

Unlocking the Gut-Lung Axis: Feixin Decoction as a Novel Modulator in Hypoxic Pulmonary Hypertension

USP22/BRD4 mediated hedgehog pathway activation contributes to airway remodeling in asthma

Macrophage-specific PHGDH protects against MAFLD by suppressing TAK1

Hepatic Abnormal Secretion of Apolipoprotein C3 Promotes Inflammation in Aortic Dissection

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Degraded products generated by iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1

PDPN+ cancer-associated fibroblasts enhance gastric cancer angiogenesis via AKT/NF-κB activation and the CCL2-ACKR1 axis

Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

Cytological Effects of Cadmium Poisoning and the Protective Effect of Quercetin: A Mechanism Exploration based on the Testicular Lamina Propria

Lactucin reverses liver fibrosis by inhibiting TGF-β1/STAT3 signaling pathway and regulating short-chain fatty acids metabolism

MFN2-dependent mitochondrial dysfunction contributes to Relm-β-induced pulmonary arterial hypertension via USP18/Twist1/miR-214 pathway

Microbial-derived Urolithin A targets GLS1 to inhibit glutaminolysis and attenuate cirrhotic portal hypertension

Heteryunine A, an amidated tryptophan-catechin-spiroketal hybrid with antifibrotic activity from Heterosmilax yunnanensis

High Hydrostatic Pressure Exacerbates Bladder Fibrosis through Activating Piezo1

Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway

Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model

Dysregulated VEGF/VEGFR-2 Signaling and Plexogenic Lesions in the Embryonic Lungs of Chickens Predisposed to Pulmonary Arterial Hypertension

Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis

Hydrogen sulfide regulates macrophage polarization and necroptosis to accelerate diabetic skin wound healing

Innovative approach: utilizing silver nanoparticles sheet for improved rabbit cecal anastomosis healing

RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway

Signal Transducer and Activator of Transcription 3/MicroRNA-21 Feedback Loop Contributes to Atrial Fibrillation by Promoting Atrial Fibrosis in a Rat Sterile Pericarditis Model

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