SKU MA1107
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number DM1A
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A)
SKU/Catalog Number MA1107
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for alpha-Tubulin detection. Tested with WB, IHC-P in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1107)
Host Mouse
Immunogen Microtubules from chicken embryo brain.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, chicken, By Heat
Western blot, 2μg/ml, Human, mouse, rat, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1107 1 IHC anti alpha tubulin antibody monoclonal dm1a.jpg

Anti-alpha-Tubulin antibody (monoclonal), MA1107, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q71U36
Gene Name TUBA1A
Protein Name Tubulin alpha-1A chain
Tissue Specificity Expressed at a high level in fetal brain. .
Alternative Names Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1A;TUBA3;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 50136 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Background Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Order Product (MA1107)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

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Publications

Zhou H, Wu G, Ma X, Xiao J, Yu G, Yang C, Xu N, Zhang B, Zhou J, Ye Z, Wang Z. J Exp Clin Cancer Res. 2018 Apr 27;37(1):89. doi: 10.1186/s13046-018-0764-9. Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse hypoxia-regulated pathways
Zhang X, Wu N. Drug Des Devel Ther. 2018 Feb 23;12:373-381. doi: 10.2147/DDDT.S147547. eCollection 2018. Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells
Schwartz, K., & Bochkariov, D. (2017). Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity. Electrophoresis. Advance online publication. doi: 10.1002/elps.201700143
Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.
Zuo Q, Liu J, Zhang J, Wu M, Guo L, Liao W. Sci Rep. 2015 Jun 25;5:11634. Doi: 10.1038/Srep11634. Development Of Trastuzumab-Resistant Human Gastric Carcinoma Cell Lines And Mechanisms Of Drug Resistance.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tuba1a antibody, tuba4a antibody