|Validated Species:||Chicken, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A)|
|Description||Mouse IgG monoclonal antibody for alpha-Tubulin detection. Tested with WB, IHC-P in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A) DM1A (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1107)|
|Validated Species||Chicken, Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Microtubules from chicken embryo brain.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tubulin alpha-1A chain|
|Molecular Weight||50136 MW|
|Protein Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.|
|Tissue Specificity||Expressed at a high level in fetal brain. .|
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Alternative Names||Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1A;TUBA3;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microtubules|tubulin||
Background for Tubulin alpha-1A chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, chicken, By Heat
Western blot, 2μg/ml, Human, mouse, rat, chicken
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A) Images
Click the images to enlarge.
IHC(P): Human Mammary Cancer Tissue