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Product Info Summary
| SKU: | M08382-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
SKU/Catalog Number
M08382-1
BM3885 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody catalog # M08382-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08382-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human alpha Tubulin
Reactive Species
M08382-1 is reactive to TUBA1B in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
49.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08382-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human A549 whole cell, human K562 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
IHC: human bladder tissue
ICC/IF: 293 cell
Validation Images & Assay Conditions
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Western blot analysis of Alpha Tubulin using anti-Alpha Tubulin antibody (M08382-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Tubulin antigen affinity purified monoclonal antibody (Catalog # M08382-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Tubulin at approximately 55 kDa. The expected band size for Alpha Tubulin is at 55 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.
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Immunofluorescent analysis of 293 cells, using alpha Tubulin Antibody .
Specific Publications For Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody (M08382-1)
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Customer Q&As
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6 Customer Q&As for Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
Question
My question regarding product M08382-1, anti-alpha Tubulin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08382-1 anti-alpha Tubulin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
Does anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 work on primate IHC with keratinocyte?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Our lab technicians have not validated anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate keratinocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-05
Question
Is a blocking peptide available for product anti-alpha Tubulin Rabbit Monoclonal antibody (M08382-1)?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We do provide the blocking peptide for product anti-alpha Tubulin Rabbit Monoclonal antibody (M08382-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-11-01
Question
I was wanting to use your anti-alpha Tubulin Rabbit Monoclonal antibody for IHC for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
It shows on the product datasheet, M08382-1 anti-alpha Tubulin Rabbit Monoclonal antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-25
Question
I am looking for to test anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 on mouse placenta for research purposes, then I may be interested in using anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Carter
Verified customer
Asked: 2016-11-03
Answer
The products we sell, including anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-03
Question
I see that the anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 works with IHC, what is the protocol used to produce the result images on the product page?
B. Taylor
Verified customer
Asked: 2013-07-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-07-26
