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- Table of Contents
TUBA1B antibody (M08382) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 18 Publications
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Immunofluorescent analysis of Hela cells, using alpha Tubulin Antibody .
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Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
TUBA1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
TUBA1B antibody was positively detected by WB in:
HeLa cell, HepG2 cell, NIH/3T3 cell, Mouse brain, C6 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TUBA1B antibody was positively detected by IHC in:
human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Human
Tested
Recommend
Recommend
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Recommend
Rat
Recommend
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Recommend
Product Information
Product Name
Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
View all TUBA1B Antibodies
View all TUBA1B Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human alpha Tubulin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
49.9 kDa
Cite This Product
Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08382)
Protocols
Western Blot protocol for TUBA1B antibody
Immunohistochemistry (IHC) protocol for TUBA1B antibody
Publications
Application:
Species:
Zhu J,Lv Y,Hao J,Shi T,Wang S,Wang K,Fan X,Guo Y,Zhang J,Li J.N-myc downstream-regulated gene 2 promotes the protein stability of estrogen receptor beta via inhibition of ubiquitin-protein ligase E3A to suppress colorectal cancer.J Gastrointest Oncol.2020
Species: Human,Mouse
PMID: 33456993
Yuan Y,Li B,Kuang Y,Ni S,Zhuge A,Yang J,Lv L,Gu S,Yan R,Li Y,Wang K,Yang L,Zhu X,Wu J,Bian X,Li L. The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell Int.2020 Jun 3;20:212.doi:10. 1186/s12935-020-01305-9
Species: Human,Mouse
PMID: 32518521
Ni S,Kuang Y,Yuan Y,Yu B. Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcoma. Cancer Cell Int.2020 Aug 18;20:399. doi:10.1186/s12935-020-01494-3.PMID:32831652;PMCID:PMC743701
Species: Human,Mouse
PMID: 32831652
CpG Oligodeoxynucleotides Downregulate Placental Adiponectin and Increase Embryo Loss in Non%u2010Obese Diabetic Mice
PMID: 27094728
Validation of Bmi1 as a therapeutic target of hepatocellular carcinoma in mice
PMID: 25372945
Stem cell autocrine CXCL12/CXCR4 stimulates invasion and metastasis of esophageal cancer
PMID: 28193907
Prognostic relevance of melanoma antigen D1 expression in colorectal carcinoma
PMID: 22935435
Baclofen protects primary rat retinal ganglion cells from chemical hypoxia-induced apoptosis through the Akt and PERK pathways
PMID: 27867349
NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder
PMID: 19412742
Prohibitin protects against oxidative stress-induced cell injury in cultured neonatal cardiomyocyte
PMID: 18958584
Endogenous Nodal promotes melanoma undergoing epithelial-mesenchymal transition via Snail and Slug in vitro and in vivo.
PMID: 26269769
Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells
PMID: 26811691
The Signaling Mechanism of TGF-?1 Induced Bovine Mammary Epithelial Cell Apoptosis
PMID: 25049567
Zhou H, Wu G, Ma X, Xiao J, Yu G, Yang C, Xu N, Zhang B, Zhou J, Ye Z, Wang Z. J Exp Clin Cancer Res. 2018 Apr 27;37(1):89. doi: 10.1186/s13046-018-0764-9. Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse ...
PMID: 29699590
Han L, Zhao Q, Liang X, Wang X, Zhang Z, Ma Z, Zhao M, Wang A, Liu S. Oncotarget. 2017 Jul 11;8(28):45643-45655. doi: 10.18632/oncotarget.17314. Ubenimex enhances Brd4 inhibition by suppressing HEXIM1 autophagic degradation and suppressing the Akt...
PMID: 28484091
Zhang X, Wu N. Drug Des Devel Ther. 2018 Feb 23;12:373-381. doi: 10.2147/DDDT.S147547. eCollection 2018. Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells
PMID: 29503530
Zuo Q, Liu J, Zhang J, Wu M, Guo L, Liao W. Sci Rep. 2015 Jun 25;5:11634. Doi: 10.1038/Srep11634. Development Of Trastuzumab-Resistant Human Gastric Carcinoma Cell Lines And Mechanisms Of Drug Resistance.
PMID: 26108989
Hu K, Xu C, Ni H, Xu Z, Wang Y, Xu S, Ji K, Xiong J, Liu H. Mol Biol Rep. 2014 Jul;41(7):4803-16. Doi: 10.1007/S11033-014-3351-Y. Epub 2014 Apr 3. Mir-218 Contributes To The Transformation Of 5-Aza/Gf Induced Umbilical Cord Mesenchymal Stem Cells ...
PMID: 24696000
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Product Promise
We promise Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M08382
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026