Anti-AMID AIFM2 Antibody

AMID antibody

Boster Bio Anti-AMID AIFM2 Antibody catalog # A06541-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Product Info Summary

SKU: A06541-1
Size: 100μl
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-AMID AIFM2 Antibody

View all AMID Antibodies

SKU/Catalog Number

A06541-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-AMID AIFM2 Antibody catalog # A06541-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMID AIFM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06541-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2B4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human AMID. at AA rangle: 110-190

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06541-1 is reactive to Aifm2 in Human, Monkey, Mouse

Applications

A06541-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

40.635kDa

Background of AMID

Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Aifm2 (Source: Uniprot.org, NCBI)

Gene Name

Aifm2

Full Name

Ferroptosis suppressor protein 1

Weight

40.635kDa

Superfamily

FAD-dependent oxidoreductase family

Alternative Names

AMID; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; apoptosis-inducing factor, mitochondrion-associated, 2; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; DKFZp686L1298; FLJ14497; p53-responsive gene 3 protein; PRG3apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer ofdeath; RP11-367H5.2 Aifm2|5430437E11Rik, Am, Amid, D730001I10Rik, FSP1, PR, PRG3|apoptosis-inducing factor, mitochondrion-associated 2|ferroptosis suppressor protein 1|apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death|apoptosis-inducing factor 2|apoptosis-inducing factor homologous mitochondrion-associated inducer of death|apoptosis-inducing factor-like mitchondrion-associated inducer of death|apoptosis-inducing factor-like mitochondrion-associated inducer of death|p53-responsive gene 3 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Aifm2, check out the Aifm2 Infographic

Aifm2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Aifm2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-AMID AIFM2 Antibody

Question

We are currently using anti-AMID antibody A06541-1 for monkey tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-22

Answer

The anti-AMID antibody (A06541-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-22

Question

See below the WB image, lot number and protocol we used for embl:aah23601.1} using anti-AMID antibody A06541-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-06-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-05

Question

I was wanting to use your anti-AMID antibody for WB for mouse embl:aah23601.1} on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse embl:aah23601.1} identification?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

It shows on the product datasheet, A06541-1 anti-AMID antibody has been validated for WB on human, monkey, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse embl:aah23601.1} in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-19

Order DetailsPrice
A06541-1

100uL

$399

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