Product Info Summary
SKU: | M06676 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody
SKU/Catalog Number
M06676
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody (Catalog # M06676). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06676)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: AMPD3/901
Isotype
IgG2b, kappa
Immunogen
Recombinant full-length human AMDP3 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06676 is reactive to AMPD3 in Human
Applications
M06676 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
88.812kDa
Background of AMPD3
This monoclonal antibody recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This monoclonal antibody shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This monoclonal antibody is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-AMPD3 Mouse Monoclonal Antibody (AMPD3/901)
Click image to see more details
Formalin-fixed, paraffin-embedded human Placenta stained with Anti-AMPD3 Mouse Monoclonal Antibody (AMPD3/901)
Protein Target Info & Infographic
Gene/Protein Information For AMPD3 (Source: Uniprot.org, NCBI)
Gene Name
AMPD3
Full Name
AMP deaminase 3
Weight
88.812kDa
Superfamily
metallo-dependent hydrolases superfamily
Alternative Names
adenosine monophosphate deaminase (isoform E); adenosine monophosphate deaminase 3; AMP aminohydrolase; AMP deaminase 3; AMP deaminase isoform E; EC 3.5.4.6; Erythrocyte AMP deaminase; erythrocyte type AMP deaminase; erythrocyte-specific AMP deaminase; myoadenylate deaminase AMPD3 adenosine monophosphate deaminase 3 AMP deaminase 3|AMP aminohydrolase|adenosine monophosphate deaminase (isoform E)|erythrocyte AMP deaminase|erythrocyte type AMP deaminase|erythrocyte-specific AMP deaminase|myoadenylate deaminase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMPD3, check out the AMPD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMPD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody (M06676)
Hello CJ!
No publications found for M06676
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal Antibody
Question
I see that the anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody M06676 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-13
Question
See attached the WB image, lot number and protocol we used for erythroleukemia using anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody M06676. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
Is there a BSA free version of anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody M06676 available?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody M06676 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-16
Question
Does M06676 anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
As indicated on the product datasheet, M06676 anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-03
Question
I have a question about product M06676, anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Patel
Verified customer
Asked: 2018-09-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06676 anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-06
Question
We are currently using anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody M06676 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
The anti-AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Monoclonal antibody (M06676) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-06