Anti-Amyloid-beta A4 protein APP Antibody

Product Name

Anti-Amyloid-beta A4 protein APP Antibody

View all APP Antibodies

SKU/Catalog Number

A00081

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Amyloid-beta A4 protein APP Antibody (Catalog # A00081). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

APP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Amyloid-beta A4 protein APP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00081)

Host

Rabbit

Contents

APP Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

APP antibody was raised against a peptide corresponding to 10 amino acids of human amyloid A4 protein precursor (APP) corresponding to the amino terminus of the 4K Ab peptide generated by beta- and gamma-secretases. The immunogen is located within amino acids 650 - 700 of APP.

Reactive Species

A00081 is reactive to APP in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

86.9 kDa

Background of APP

Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00081 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APP antibody can be used for detection of APP and Ab peptide by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information APP

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