Product Info Summary
SKU: | A00542 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Androgen Receptor/AR Antibody Picoband™
View all Androgen R/NR3C4 Antibodies
SKU/Catalog Number
A00542
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Androgen Receptor/AR Antibody Picoband™ catalog # A00542. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Androgen Receptor/AR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00542)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Androgen Receptor/AR recombinant protein (Position: A629-Q920).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00542 is reactive to AR in Human, Mouse, Rat
Applications
A00542 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
99.188kDa
Background of Androgen R/NR3C4
The AR (androgen receptor) gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The AR gene is mapped to Xq12. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human 22RV1 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human CACO-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen Receptor/AR antigen affinity purified polyclonal antibody (Catalog # A00542) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Androgen Receptor/AR at approximately 120KD. The expected band size for Androgen Receptor/AR is at 120KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of A549 cells using anti-Androgen Receptor/AR antibody (A00542).
Overlay histogram showing A549 cells stained with A00542 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Androgen Receptor/AR Antibody (A00542, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of C6 cells using anti-Androgen Receptor/AR antibody (A00542).
Overlay histogram showing C6 cells stained with A00542 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Androgen Receptor/AR Antibody (A00542, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. Flow Cytometry analysis of RAW264.7 cells using anti-Androgen Receptor/AR antibody (A00542).
Overlay histogram showing RAW264.7 cells stained with A00542 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Androgen Receptor/AR Antibody (A00542, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 5. IF analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542).
Androgen Receptor/AR was detected in immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Androgen Receptor/AR Antibody (A00542) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For AR (Source: Uniprot.org, NCBI)
Gene Name
AR
Full Name
Androgen receptor
Weight
99.188kDa
Superfamily
nuclear hormone receptor family
Alternative Names
AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy AR AIS8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, AR androgen receptor androgen receptor|dihydrotestosterone receptor|nuclear receptor subfamily 3 group C member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AR, check out the AR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Androgen Receptor/AR Antibody Picoband™ (A00542)
Hello CJ!
A00542 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of extract of Buddleja officinalis eye drops on androgen receptors of lacrimal gland cells of castrated rats with dry eye
Elevated expression levels of androgen receptors and matrix metalloproteinase-2 and -9 in 30 cases of hepatocellular carcinoma compared with adjacent tissues as predictors of cancer invasion and staging
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