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- Table of Contents
ANGPT2 antibody (A00370-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Flow Cytometry analysis of HeLa cells using anti-ANGPT2 antibody (A00370-2).
Overlay histogram showing HeLa cells stained with A00370-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody (A00370-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HeLa cells stained with A00370-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody (A00370-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Angiopoietin-2/ANGPT2 Antibody Picoband®
ANGPT2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ANGPT2 antibody was positively detected by WB in:
human SW620 whole cell, human A549 whole cell, human K562 whole cell, rat brain tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ANGPT2 antibody was positively detected by IHC in:
human mammary cancer tissue, human placenta tissue, human rectal cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ANGPT2 antibody was positively detected by ICC/IF in:
A549 cell
ANGPT2 antibody was positively detected by FC in:
HeLa cell, U937 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Angiopoietin-2/ANGPT2 Antibody Picoband®
View all ANGPT2 Antibodies
View all ANGPT2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Angiopoietin-2/ANGPT2 recombinant protein (Position: N357-K404).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
66 kDa
Calculated Molecular Weight
56.9 kDa
Background of ANGPT2
ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Cite This Product
Anti-Angiopoietin-2/ANGPT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00370-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
96%
High Homology
Cow
88%
Moderate Homology
Sheep
88%
Moderate Homology
Mouse
81%
Moderate Homology
Rat
81%
Moderate Homology
Zebrafish
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANGPT2 antibody
Immunohistochemistry (IHC) protocol for ANGPT2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ANGPT2 antibody
Flow Cytometry (FC) protocol for ANGPT2 antibody
Publications
Application:
Species:
Comprehensive analysis and validation of angiogenesis in vascular dementia from the perspective of diagnosis, prevention, and treatment
Published: 2025-09-08
Journal: Frontiers in Genetics
Application: WB
Impact Factor: 2.80
Species: Rat
PMID: 40988927
Single-cell analysis reveals the implication of vascular endothelial cell-intrinsic ANGPT2 in human intracranial aneurysm
Published: 2024-08-24
Journal: CARDIOVASCULAR RESEARCH
Application: IF
Impact Factor: 10.20
Species: Human
PMID: 39187926
An oxidation responsive nano-radiosensitizer increases radiotherapy efficacy by remolding tumor vasculature
Published: 2021-07-31
Journal: Biomaterials Science
Application: WB
Impact Factor: 6.84
Species: Human
PMID: 34519724
Construction of an AuHQ nano-sensitizer for enhanced radiotherapy efficacy through remolding tumor vasculature
Published: 2021-04-28
Journal: Journal of Materials Chemistry B
Application: WB
Impact Factor: 6.33
Species: Human
PMID: 34013945
Expression of angiopoietins in central nervous system hemangioblastomas is associated with cyst formation
Published: 2016-12-29
Journal: NEUROSCIENCE LETTERS
Application: WB,IF
Impact Factor: 2.11
Species: Human
PMID: 28041961
Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients
Published: 2014-12-17
Journal: OSTEOPOROSIS INTERNATIONAL
Application: IHC
Impact Factor: 4.17
Species: Human
PMID: 25516362
Antiangiogenic Treatment Diminishes Renal Injury and Dysfunction via Regulation of Local AKT in Early Experimental Diabetes
Published: 2014-04-23
Journal: PLoS One
Application: IF
Impact Factor: 3.53
Species: Rat
PMID: 24759991
The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery
Published: 2014-04-05
Journal: CARDIOVASCULAR PATHOLOGY
Application: IHC
Impact Factor: 2.34
Species: Human
PMID: 24877885
Exercise Improves Recovery after Ischemic Brain Injury by Inducing the Expression of Angiopoietin-1 and Tie-2 in Rats
Published: 2011-06-24
Journal: TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
Application: IHC
Impact Factor: 1.15
Species: Rat
PMID: 21701128
Expression of heparanase and angiopoietin-2 in patients with endometriosis
Published: 2006-12-01
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology
Application: WB,IHC
Impact Factor: 1.14
Species: Human
PMID: 17141400
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Product Promise
We promise Anti-Angiopoietin-2/ANGPT2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00370-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026