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Product Info Summary
| SKU: | A00370 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-Angiopoietin-2/ANGPT2 Antibody Picoband®
SKU/Catalog Number
A00370
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiopoietin-2/ANGPT2 Antibody Picoband® catalog # A00370. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. ANGPT2 is described as a naturally occurring antagonist of ANGPT1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation, disrupting vascular remodeling and potentially promoting endothelial apoptosis (as described on-page). Assay context: Human-reactive antibody validated for ELISA, IHC, and Western blot with reported observed band ~70 kDa (calculated 56.9 kDa) and on-page assay starting concentrations; tissue-localization interpretation can be framed within histology workflows and neutral review via pathology review (method-context).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiopoietin-2/ANGPT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00370)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Angiopoietin-2 recombinant protein (Position: E180-D283). Human Angiopoietin-2 shares 75% and 77.9% amino acid (aa) sequence identity with mouse and rat Angiopoietin-2, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00370 is reactive to ANGPT2 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
56.9 kDa
Background of ANGPT2
ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00370 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA(Cap) , 1-5μg/ml, Human, -
Positive Control
WB: human HepG2 whole cell
IHC: human lung cancer tissue, human placenta tissue
Validation Images & Assay Conditions
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IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody (A00370).
Angiopoietin-2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Angiopoietin-2 Antibody (A00370) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody (A00370).
Angiopoietin-2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Angiopoietin-2 Antibody (A00370) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (Catalog # A00370) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT2 at approximately 70KD. The expected band size for ANGPT2 is at 57KD.
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Sandwich ELISA - Recombinant human Angiopoietin-2 protein standard curve.
Use in combination with reagents from Human Angiopoietin-2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0657).
Specific Publications For Anti-Angiopoietin-2/ANGPT2 Antibody Picoband® (A00370)
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