Anti-Angiotensin II Receptor AT2 Antibody

Rabbit Polyclonal antibody for Agtr2 detection. Tested positive for ELISA, IHC, WB in Rat

SKU A00432
Size 100µg
Reactivity Rat
Clonality Polyclonal
Host Rabbit
Applications ELISA, IHC, WB

Overview

Product Name Anti-Angiotensin II Receptor AT2 Antibody
See all Agtr2 primary antibodies, ELISA kits and proteins
SKU/Catalog Number A00432
Storage & Handling -20°C
Size 100µg
Description Rabbit Polyclonal antibody for Agtr2 detection. Tested positive for ELISA, IHC, WB in Rat
Cite This Product Anti-Angiotensin II Receptor AT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00432)
Specificity Anti-Angiotensin II Receptor AT2 Antibody (A00432) reacts with Rat Agtr2, in native form and recombinant. Superfamily members of Agtr2 are not reactive to A00432.
Host Rabbit
Contents/Buffer Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Form Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Immunogen Synthetic peptide corresponding to the sequence near the N-terminus of rat angiotensin II receptor AT2.
Reactivity Rat

Assay Details

Our Boster Quality Guarantee for Anti-Angiotensin II Receptor AT2 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Optimal conditions must be determined individually for each application.

Images And Assay Conditions

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Membrane ELISA: Lewis rat kidney membranes (5µg/well) were treated with 1μM concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT2) pAb (1:250 to 1:2000 of a 1µg/μL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).

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Figure 2. IHC analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Agtr2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Agtr2 Antibody (A00432) overnight at 4?°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37?°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 1. Western blot analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Agtr2 antigen affinity purified polyclonal antibody (Catalog # A00432) at 0.5 ug/mL overnight at 4?°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Agtr2.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P35351
Gene Name Agtr2

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Rat

*You can search these to find other products in these research areas.

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In stock
Order Product
A00432
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$307.00

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