SKU A00432
Size 100µg
Reactivity Rat
Clonality Polyclonal
Host Rabbit
Applications ELISA, IHC, WB

Overview

Product Name Anti-Angiotensin II Receptor AT2 Antibody
SKU/Catalog Number A00432
Storage & Handling -20°C
Size 100µg
Description Rabbit Polyclonal antibody for Agtr2 detection. Tested positive for ELISA, IHC, WB in Rat
Cite This Product Anti-Angiotensin II Receptor AT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00432)
Host Rabbit
Contents/Buffer Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Form Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Immunogen Synthetic peptide corresponding to the sequence near the N-terminus of rat angiotensin II receptor AT2.
Reactivity Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Optimal conditions must be determined individually for each application.

Images And Assay Conditions

/A/0/A00432 Agtr2 elisa test 3.jpg

Membrane ELISA: Lewis rat kidney membranes (5µg/well) were treated with 1μM concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT2) pAb (1:250 to 1:2000 of a 1µg/μL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).

/A/0/A00432 Agtr2 IHC test 2.jpg

Figure 2. IHC analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Agtr2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Agtr2 Antibody (A00432) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A00432 Agtr2 WB test 1.jpg

Figure 1. Western blot analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Agtr2 antigen affinity purified polyclonal antibody (Catalog # A00432) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Agtr2.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P35351
Gene Name Agtr2

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Apoptosis, Atherosclerosis, Cardiovascular, G Protein Signaling, Lipid Signaling, Signal Transduction, Signaling Pathway

*You can search these to find other products in these research areas.

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Order Product (A00432)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
100µg $307
100µg+Free HRP Secondary BA1054 $307
100µg+Free Biotin Secondary BA1003 $307

USD $307

Ships in 7-10 business days.

Troubleshooting

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