Anti-Angiotensin II Receptor AT2 Antibody
Rabbit Polyclonal antibody for Agtr2 detection. Tested positive for ELISA, IHC, WB in Rat
|Applications||ELISA, IHC, WB|
|Product Name||Anti-Angiotensin II Receptor AT2 Antibody
See all Agtr2 primary antibodies, ELISA kits and proteins
|Storage & Handling||-20°C|
|Description||Rabbit Polyclonal antibody for Agtr2 detection. Tested positive for ELISA, IHC, WB in Rat|
|Cite This Product||Anti-Angiotensin II Receptor AT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00432)|
|Specificity||Anti-Angiotensin II Receptor AT2 Antibody (A00432) reacts with Rat Agtr2, in native form and recombinant. Superfamily members of Agtr2 are not reactive to A00432.|
|Contents/Buffer||Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.|
|Form||Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.|
|Immunogen||Synthetic peptide corresponding to the sequence near the N-terminus of rat angiotensin II receptor AT2.|
Our Boster Quality Guarantee for Anti-Angiotensin II Receptor AT2 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Optimal conditions must be determined individually for each application.
Images And Assay Conditions
Membrane ELISA: Lewis rat kidney membranes (5Âµg/well) were treated with 1Î¼M concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT2) pAb (1:250 to 1:2000 of a 1Âµg/Î¼L stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean Â± SEM (n=2).
Figure 2. IHC analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Agtr2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Agtr2 Antibody (A00432) overnight at 4?Â°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37?Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Agtr2 antigen affinity purified polyclonal antibody (Catalog # A00432) at 0.5 ug/mL overnight at 4?Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Agtr2.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
*You can search these to find other products in these research areas.
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