SKU A00432
Size 100µg
Reactivity Rat
Clonality Polyclonal
Host Rabbit
Applications ELISA, IHC, WB


Product Name Anti-Angiotensin II Receptor AT2 Antibody
SKU/Catalog Number A00432
Storage & Handling -20°C
Size 100µg
Description Rabbit Polyclonal antibody for Agtr2 detection. Tested positive for ELISA, IHC, WB in Rat
Cite This Product Anti-Angiotensin II Receptor AT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00432)
Host Rabbit
Contents/Buffer Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Form Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Immunogen Synthetic peptide corresponding to the sequence near the N-terminus of rat angiotensin II receptor AT2.
Reactivity Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Optimal conditions must be determined individually for each application.

Images And Assay Conditions

/A/0/A00432 Agtr2 elisa test 3.jpg

Membrane ELISA: Lewis rat kidney membranes (5µg/well) were treated with 1μM concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT2) pAb (1:250 to 1:2000 of a 1µg/μL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).

/A/0/A00432 Agtr2 IHC test 2.jpg

Figure 2. IHC analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Agtr2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Agtr2 Antibody (A00432) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A00432 Agtr2 WB test 1.jpg

Figure 1. Western blot analysis of Agtr2 using anti-Agtr2 antibody (A00432).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Agtr2 antigen affinity purified polyclonal antibody (Catalog # A00432) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Agtr2.

Target Info

Protein Target Info (Source:

Uniprot Id P35351
Gene Name Agtr2

*if product is indicated to react with multiple species, protein info is based on the human gene.


Research Areas Apoptosis, Atherosclerosis, Cardiovascular, G Protein Signaling, Lipid Signaling, Signal Transduction, Signaling Pathway

*You can search these to find other products in these research areas.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Order Product (A00432)


Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
100µg $307
100µg+Free HRP Secondary BA1054 $307
100µg+Free Biotin Secondary BA1003 $307

USD $307

Ships in 7-10 business days.


Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for A00432