Anti-Angiotensinogen/AGT Antibody

AGT antibody

Boster Bio Anti-Angiotensinogen/AGT Antibody catalog # PA1643. Tested in IHC, WB applications. This antibody reacts with Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: PA1643
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Angiotensinogen/AGT Antibody

View all AGT Antibodies

SKU/Catalog Number

PA1643

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiotensinogen/AGT Antibody catalog # PA1643. Tested in IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiotensinogen/AGT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1643)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Angiotensinogen, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1643 is reactive to AGT in Mouse

Applications

PA1643 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

53.154kDa

Background of AGT

AGT (ANGIOTENSINOGEN), also called SERPINA8, is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Western blot analysis showed a distinct band of expected size of the angiotensinogen protein (61 kD) in isolated adipocytes. The more pronounced AGT increase in TT subjects resulted in similar plasma renin activity at a lower plasma active renin concentration, with a higher plasma renin activity/active renin ratio.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For AGT (Source: Uniprot.org, NCBI)

Gene Name

AGT

Full Name

Angiotensinogen

Weight

53.154kDa

Superfamily

serpin family

Alternative Names

AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8); angiotensin I; angiotensin II; ANHU; SERPINA8 AGT ANHU, SERPINA8, hFLT1 angiotensinogen angiotensinogen|alpha-1 antiproteinase, antitrypsin|angiotensin I|angiotensin II|fetal-liver predominant transporter 1|pre-angiotensinogen|serine (or cysteine) proteinase inhibitor|serpin A8|serpin peptidase inhibitor, clade A, member 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AGT, check out the AGT Infographic

AGT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AGT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1643 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lan F, Yue X, Han L, Shi Z, Yang Y, Pu P, Yao Z, Kang C. Int J Oncol. 2012 Feb;40(2):519-26. Doi: 10.3892/Ijo.2011.1215. Epub 2011 Sep 28. Genome-Wide Identification Of Tcf7L2/Tcf4 Target Mirnas Reveals A Role For Mir-21 In Wnt-Driven Epithelial C...

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1 Customer Q&As for Anti-Angiotensinogen/AGT Antibody

Question

We are currently using anti-Angiotensinogen/AGT antibody PA1643 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

P. Jones

Verified customer

Asked: 2013-08-30

Answer

The anti-Angiotensinogen/AGT antibody (PA1643) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-30

Size

Conjugation

Total: $370

SKU:PA1643

In stock, 2 left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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