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Product Info Summary
| SKU: | A05747-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ANGPTL2 Antibody Picoband®
SKU/Catalog Number
A05747-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ANGPTL2 Antibody Picoband® catalog # A05747-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANGPTL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05747-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ANGPTL2, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A05747-1 is reactive to ANGPTL2 in Human, Mouse, Rat
Observed Molecular Weight
60-65 kDa
Calculated molecular weight
57.1 kDa
Background of ANGPTL2
Angiopoietin-related protein 2, also known as angiopoietin-like protein 2, is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05747-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human HUVEC whole cell, human SH-SY5Y whole cell, rat heart tissue, mouse heart tissue
IHC: human colon cancer tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of ANGPTL2 using anti-ANGPTL2 antibody (A05747-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HUVEC whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL2 antigen affinity purified polyclonal antibody (A05747-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ANGPTL2 at approximately 60-65 kDa. The expected band size for ANGPTL2 is at 57 kDa.
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IHC analysis of ANGPTL2 using anti-ANGPTL2 antibody (A05747-1).
ANGPTL2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPTL2 Antibody (A05747-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of ANGPTL2 using anti-ANGPTL2 antibody (A05747-1).
ANGPTL2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPTL2 Antibody (A05747-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-ANGPTL2 Antibody Picoband® (A05747-1)
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Customer Q&As
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7 Customer Q&As for Anti-ANGPTL2 Antibody Picoband®
Question
See below the WB image, lot number and protocol we used for tendon of biceps brachii using anti-ANGPTL2 antibody A05747-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-15
Question
Do you have a BSA free version of anti-ANGPTL2 antibody A05747-1 available?
T. Yang
Verified customer
Asked: 2018-06-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ANGPTL2 antibody A05747-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tendon of biceps brachii using anti-ANGPTL2 antibody A05747-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-12
Question
Would anti-ANGPTL2 antibody A05747-1 work on canine IHC with tendon of biceps brachii?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
Our lab technicians have not tested anti-ANGPTL2 antibody A05747-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ANGPTL2 antibody A05747-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine tendon of biceps brachii in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-09
Question
We are currently using anti-ANGPTL2 antibody A05747-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
The anti-ANGPTL2 antibody (A05747-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-31
Question
I see that the anti-ANGPTL2 antibody A05747-1 works with WB, what is the protocol used to produce the result images on the product page?
F. Li
Verified customer
Asked: 2017-07-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-07
Question
Is a blocking peptide available for product anti-ANGPTL2 antibody (A05747-1)?
S. Gonzalez
Verified customer
Asked: 2016-11-07
Answer
We do provide the blocking peptide for product anti-ANGPTL2 antibody (A05747-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-11-07
