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Product Info Summary
| SKU: | A00469-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Antithrombin III/SERPINC1 Antibody Picoband®
SKU/Catalog Number
A00469-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Antithrombin III/SERPINC1 Antibody Picoband® catalog # A00469-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Antithrombin III/SERPINC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00469-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Antithrombin III/SERPINC1 recombinant protein (Position: R89-K464).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00469-1 is reactive to SERPINC1 in Human, Mouse, Rat
Observed Molecular Weight
53 kDa
Calculated molecular weight
52.6 kDa
Background of SERPINC1
Serpin peptidase inhibitor, clade C(antithrombin), member 1, also known as Antithrombin III(AT3) is the most important inhibitor of thrombin OMBIN III and other coagulation proteinases. This gene is mapped to 1q25.1. The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00469-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat liver tissue, mouse testis tissue, mouse ovary tissue, mouse liver tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of Antithrombin III/SERPINC1 using anti-Antithrombin III/SERPINC1 antibody (A00469-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse testis tissue lysates,
Lane 3: mouse ovary tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Antithrombin III/SERPINC1 antigen affinity purified polyclonal antibody (Catalog # A00469-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Antithrombin III/SERPINC1 at approximately 53 kDa. The expected band size for Antithrombin III/SERPINC1 is at 52 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-Antithrombin III/SERPINC1 antibody (A00469-1).
Overlay histogram showing HepG2 cells stained with A00469-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Antithrombin III/SERPINC1 Antibody (A00469-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Antithrombin III/SERPINC1 Antibody Picoband® (A00469-1)
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