Product Info Summary
SKU: | A06544-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-AP-2 complex subunit beta AP2B1 Antibody
View all Beta 2 Adaptin Antibodies
SKU/Catalog Number
A06544-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AP-2 complex subunit beta AP2B1 Antibody catalog # A06544-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AP-2 complex subunit beta AP2B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06544-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human AMID
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06544-1 is reactive to AP2B1 in Human, Mouse, Rat
Applications
A06544-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
104.553kDa
Background of Beta 2 Adaptin
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
Ross M.T., Nature 434:325-337(2005
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For AP2B1 (Source: Uniprot.org, NCBI)
Gene Name
AP2B1
Full Name
AP-2 complex subunit beta
Weight
104.553kDa
Superfamily
adaptor complexes large subunit family
Alternative Names
Adapter-related protein complex 2 beta subunit; Adaptor protein complex AP-2 subunit beta; adaptor-related protein complex 2, beta 1 subunit; ADTB2adaptin, beta 2 (beta); AP105B; AP2-BETA; beta-2-adaptin; beta-adaptin; CLAPB1AP-2 complex subunit beta; Clathrin assembly protein complex 2 beta large chain; clathrin-associated/assembly/adaptor protein, large, beta 1; DKFZp781K0743; Plasma membrane adaptor HA2/AP2 adaptin beta subunit AP2B1 ADTB2, AP105B, AP2-BETA, CLAPB1 adaptor related protein complex 2 subunit beta 1 AP-2 complex subunit beta|adapter-related protein complex 2 beta subunit|adapter-related protein complex 2 subunit beta|adaptin, beta 2 (beta)|adaptor protein complex AP-2 subunit beta|adaptor related protein complex 2 beta 1 subunit|adaptor-related protein complex 2 subunit beta|beta-2-adaptin|beta-adaptin|beta2-adaptin|clathrin assembly protein complex 2 beta large chain|clathrin-associated/assembly/adaptor protein, large, beta 1|plasma membrane adaptor HA2/AP2 adaptin beta subunit|testicular tissue protein Li 22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP2B1, check out the AP2B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP2B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP-2 complex subunit beta AP2B1 Antibody (A06544-1)
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No publications found for A06544-1
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