Anti-AP2B1 Antibody Picoband®

Product Name

Anti-AP2B1 Antibody Picoband®

View all AP2B1 Antibodies

SKU/Catalog Number

A06544

Size

100 μg/vial

Form

Lyophilized

Description

AP2B1 is a large subunit of the AP-2 adaptor complex central to clathrin-mediated endocytosis, linking clathrin coats to membrane cargo receptors (GO:0030119 clathrin-coated vesicle; vesicle-mediated transport). Assay context: antibody tested for Western blotting (per product listing); functionally relevant to receptor internalization pathways that intersect with lipid metabolism (e.g., LDLR trafficking impacted by PCSK9, putative) and can be paired with transporter readouts such as ABCG4 in membrane-traffic studies (putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AP2B1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06544)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human AP2B1 recombinant protein (Position: Q311-R522). Human AP2B1 shares 100% amino acid (aa) sequence identity with both mouse and rat AP2B1.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06544 is reactive to AP2B1 in Human, Mouse, Rat

Observed Molecular Weight

105 kDa

Calculated molecular weight

104.6 kDa

Background of AP2B1

AP-2 complex subunit beta is a protein that in humans is encoded by the AP2B1 gene. It is mapped to 17q12. The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06544 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: human A549 whole cell, human K562 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue

Validation Images & Assay Conditions

See full target information AP2B1

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