Anti-AP-2 complex subunit mu AP2M1 Antibody

Product Name

Anti-AP-2 complex subunit mu AP2M1 Antibody

View all AP2M1 Antibodies

SKU/Catalog Number

A06179-1

Size

0.1 mg

Form

Liquid

Description

AP2M1 (AP-2 complex subunit mu) is part of the AP-2 adaptor complex central to clathrin-dependent endocytosis and receptor/cargo internalization; it is also described as influencing endosome/lysosome acidification (via v-ATPase activity) and intracellular trafficking of immune regulators such as CTLA-4 (context-dependent). Assay context: antibody raised against a short synthetic peptide near the C-terminus of human AP2M1; validated in ELISA, WB, IHC-P, and IF, supporting studies of membrane trafficking, receptor internalization, and vesicular transport in IHC or Western blotting readouts. Often contextualized with immune-activation markers (e.g., IL-18) when linking trafficking to immunoregulatory pathways.

Storage & Handling

AP2M1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-AP-2 complex subunit mu AP2M1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06179-1)

Host

Chicken

Contents

AP2M1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgY

Immunogen

AP2M1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human AP2M1. The immunogen is located within amino acids 360 - 410 of AP2M1.

Reactive Species

A06179-1 is reactive to AP2M1 in Human, Mouse, Rat

Calculated molecular weight

49.7 kDa

Background of AP2M1

The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06179-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AP2M1

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