Anti-AP-3 complex subunit beta-2 AP3B2 Antibody

Product Name

Anti-AP-3 complex subunit beta-2 AP3B2 Antibody

View all AP3B2 Antibodies

SKU/Catalog Number

A09539

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AP-3 complex subunit beta-2 AP3B2 Antibody (Catalog # A09539). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-AP-3 complex subunit beta-2 AP3B2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09539)

Host

Rabbit

Contents

AP3B2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AP3B2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AP3B2. The immunogen is located within the first 50 amino acids of AP3B2.

Reactive Species

A09539 is reactive to AP3B2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

119.1 kDa

Background of AP3B2

The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Unlike the homologous AP3B1, AP3B2-containing AP3 complexes are preferentially targeted to neuronal processes, and mice deficient in AP3B2 displayed compromised synaptic zinc stores.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09539 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AP3B2 antibody can be used for detection of AP3B2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AP3B2

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