Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody

Product Name

Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody

View all AP3S1 Antibodies

SKU/Catalog Number

A12249

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody (Catalog # A12249). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

AP3S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12249)

Host

Rabbit

Contents

AP3S1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AP3S1 antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1. The immunogen is located within the first 50 amino acids of AP3S1.

Cross-reactivity

At least three isoforms of AP3S1 are known to exist; this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members.

Reactive Species

A12249 is reactive to AP3S1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

21.7 kDa

Background of AP3S1

AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12249 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AP3S1 antibody can be used for detection of AP3S1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AP3S1

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