Product Info Summary
SKU: | A10072-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, IHC-P, WB, IHC-P-Leica |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AP1M1 Antibody (Center)
SKU/Catalog Number
A10072-1
Size
80 µl
Description
Boster Bio Anti-AP1M1 Antibody (Center) (Catalog # A10072-1). Tested in WB, IF, IHC-P, IHC-P-Leica application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AP1M1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10072-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-227 amino acids from the Central region of human AP1M1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10072-1 is reactive to AP1M1 in Human, Mouse
Applications
A10072-1 is guaranteed for IF, IHC-P, WB, IHC-P-Leica Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48.587kDa
Background of AP1M1
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
Click image to see more details
Confocal immunofluorescent analysis of AP1M1 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Click image to see more details
Anti-AP1M1 Antibody (Center) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 49 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-AP1M1 Antibody (Center) at 1:2000 dilution
Lane 1: PC-3 whole cell lysate
Lane 2: U-251 MG whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: Mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 49 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody (Center). A10072-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Click image to see more details
AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human brain tissue using A10072-1 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For AP1M1 (Source: Uniprot.org, NCBI)
Gene Name
AP1M1
Full Name
AP-1 complex subunit mu-1
Weight
48.587kDa
Superfamily
adaptor complexes medium subunit family
Alternative Names
AP-1 complex subunit mu-1 AP1M1 AP47, CLAPM2, CLTNM, MU-1A, mu1A adaptor related protein complex 1 subunit mu 1 AP-1 complex subunit mu-1|AP-mu chain family member mu1A|HA1 47 kDa subunit|adapter-related protein complex 1 subunit mu-1|adaptor protein complex AP-1 mu-1 subunit|adaptor protein complex AP-1 subunit mu-1|adaptor related protein complex 1 mu 1 subunit|clathrin adaptor protein AP47|clathrin assembly protein complex 1 medium chain 1|clathrin assembly protein complex 1 mu-1 medium chain 1|clathrin assembly protein complex 1, medium chain|clathrin assembly protein complex AP1, mu subunit|clathrin coat assembly protein AP47|clathrin coat-associated protein AP47|golgi adaptor AP-1 47 kDa protein|golgi adaptor HA1/AP1 adaptin mu-1 subunit|mu-adaptin 1|mu1A-adaptin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP1M1, check out the AP1M1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP1M1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP1M1 Antibody (Center) (A10072-1)
Hello CJ!
No publications found for A10072-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AP1M1 Antibody (Center)?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-AP1M1 Antibody (Center)
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question