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Product Info Summary
| SKU: | A10072-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB, IHC-P-Leica |
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Product info
Product Name
Anti-AP1M1 Antibody (Center)
SKU/Catalog Number
A10072-1
Size
80 µl
Description
Boster Bio Anti-AP1M1 Antibody (Center) (Catalog # A10072-1). Tested in WB, IF, IHC-P, IHC-P-Leica application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AP1M1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10072-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-227 amino acids from the Central region of human AP1M1.
Reactive Species
A10072-1 is reactive to AP1M1 in Human, Mouse
Calculated molecular weight
48.6 kDa
Background of AP1M1
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10072-1 is guaranteed for IF, IHC-P, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of AP1M1 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Anti-AP1M1 Antibody (Center) at 1:2000 dilution + Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 49 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-AP1M1 Antibody (Center) at 1:2000 dilution
Lane 1: PC-3 whole cell lysate
Lane 2: U-251 MG whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: Mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 49 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody (Center). A10072-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using A10072-1 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-AP1M1 Antibody (Center) (A10072-1)
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