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Product Info Summary
| SKU: | M06179 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06179
BM4613 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody catalog # M06179. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06179)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GDD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AP2M1
Reactive Species
M06179 is reactive to AP2M1 in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
49.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06179 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: HEK293 cell, MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of AP2M1 expression in (1) HEK293 cell lysate; (2) MCF-7 cell lysate.
Specific Publications For Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody (M06179)
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Customer Q&As
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6 Customer Q&As for Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody
Question
Would M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
As indicated on the product datasheet, M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
I was wanting to use to test anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179 on rat erythroleukemia for research purposes, then I may be interested in using anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The products we sell, including anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody reactive to the isotypes of AP2M1?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The immunogen of M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human AP2M1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-10
Question
I have a question about product M06179, anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-11
