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Product Info Summary
| SKU: | A07396-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-APBA3/Mint3 Antibody
SKU/Catalog Number
A07396-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-APBA3/Mint3 Antibody (Catalog# A07396-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APBA3/Mint3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07396-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human APBA3. Immunogen sequence location: 361-410
Reactive Species
A07396-1 is reactive to APBA3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
61.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07396-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of lysates from HUVEC cells, using APBA3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using APBA3 antibody.
Specific Publications For Anti-APBA3/Mint3 Antibody (A07396-1)
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Customer Q&As
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3 Customer Q&As for Anti-APBA3/Mint3 Antibody
Question
Is this A07396-1 anti-APBA3/Mint3 antibody reactive to the isotypes of APBA3?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
The immunogen of A07396-1 anti-APBA3/Mint3 antibody is A peptide derived from human APBA3. Immunogen sequence location: 361-410. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-17
Question
See below the WB image, lot number and protocol we used for lung using anti-APBA3/Mint3 antibody A07396-1. Please let me know if you require anything else.
M. Brown
Verified customer
Asked: 2019-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-13
Question
Can you help my question with product A07396-1, anti-APBA3/Mint3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Bhatt
Verified customer
Asked: 2014-06-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07396-1 anti-APBA3/Mint3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-06-30
