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Product Info Summary
| SKU: | A07065-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-APC10 ANAPC10 Antibody
SKU/Catalog Number
A07065-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-APC10 ANAPC10 Antibody (Catalog # A07065-1). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-APC10 ANAPC10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07065-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human APC10.
Reactive Species
A07065-1 is reactive to ANAPC10 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
21.3 kDa
Background of ANAPC10
APC (Anaphase-Promoting Complex or Cyclosome) is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B. It was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid-body in telophase and cytokinesis. These results strongly support the notion that human APC10/Doc1 may be one of the APC core subunits rather than the transiently associated regulatory factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07065-1 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:15,000 - 1:80,000
IHC: 1:250 - 1:1,000
IP: 1:100
WB: 1:500 - 1:1,500
This affinity purified antibody has been tested for use in ELISA against the immunizing peptide and by western blotting against various cell lysates. Reactivity in other immunoassays is unknown.
Validation Images & Assay Conditions
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Western Blot of Affinity Purified Rabbit anti-APC10. Lane 1: HeLa nuclear extract . Lane 2: HeLa whole cell lysate . Lane 3: A431 whole cell lysate . Lane 4: Jurkat whole cell lysate . Lane 5: 293 whole cell lysate . All lanes contain 20 µg of lysate or extract. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h to detect human APC10 at ~26 kDa (predicted molecular weight is 21 kDa). After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
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Western Blot of Affinity Purified Rabbit Anti-APC10. Lane 1: HeLa whole cell lysate . Lane 2: HeLa Nuclear lysate . Approximately 20 µg of each lysate was loaded onto a 10% SDS-PAGE gel. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa.
Specific Publications For Anti-APC10 ANAPC10 Antibody (A07065-1)
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3 Customer Q&As for Anti-APC10 ANAPC10 Antibody
Question
Will anti-APC10 antibody A07065-1 work on monkey WB with brain?
E. Banerjee
Verified customer
Asked: 2018-01-03
Answer
Our lab technicians have not validated anti-APC10 antibody A07065-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-APC10 antibody A07065-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-03
Question
We are currently using anti-APC10 antibody A07065-1 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
The anti-APC10 antibody (A07065-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-02
Question
I was wanting to use your anti-APC10 antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human erythroleukemia identification?
G. Huang
Verified customer
Asked: 2015-03-10
Answer
You can see on the product datasheet, A07065-1 anti-APC10 antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-10
