Product Info Summary
SKU: | A14554 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-APC13 ANAPC13 Antibody
SKU/Catalog Number
A14554
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC13 ANAPC13 Antibody (Catalog # A14554). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC13 ANAPC13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14554)
Host
Rabbit
Contents
APC13 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13. The immunogen is located within the last 50 amino acids of APC13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A14554 is reactive to ANAPC13 in Human, Mouse, Rat
Applications
A14554 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
8.521kDa
Background of ANAPC13
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC13 antibody can be used for detection of APC13 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APC13 in Jurkat cell tissue lysate with APC13 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of APC13 in Jurkat cells with APC13 antibody at 20 μg/mL.
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Western blot analysis with 25ng of recombinant APC13 protein with APC13 antibody at 1 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For ANAPC13 (Source: Uniprot.org, NCBI)
Gene Name
ANAPC13
Full Name
Anaphase-promoting complex subunit 13
Weight
8.521kDa
Superfamily
APC13 family
Alternative Names
Anaphase-promoting complex subunit 13 ANAPC13 APC13, SWM1 anaphase promoting complex subunit 13 anaphase-promoting complex subunit 13|cyclosome subunit 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANAPC13, check out the ANAPC13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANAPC13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC13 ANAPC13 Antibody (A14554)
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