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Product Info Summary
| SKU: | M00820 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody
SKU/Catalog Number
M00820
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody catalog # M00820. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00820)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABID-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Apg12 (Atg12)
Reactive Species
M00820 is reactive to ATG12 in Human
Observed Molecular Weight
20, 55 kDa
Calculated molecular weight
15.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00820 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Apg12 expression in HepG2 cell lysate.
Specific Publications For Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody (M00820)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody
Question
We are currently using anti-Apg12 (Atg12) Rabbit Monoclonal antibody M00820 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The anti-Apg12 (Atg12) Rabbit Monoclonal antibody (M00820) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-27
Question
Is a blocking peptide available for product anti-Apg12 (Atg12) Rabbit Monoclonal antibody (M00820)?
K. Anderson
Verified customer
Asked: 2019-08-06
Answer
We do provide the blocking peptide for product anti-Apg12 (Atg12) Rabbit Monoclonal antibody (M00820). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-06
Question
Here is the WB image, lot number and protocol we used for brain ovary using anti-Apg12 (Atg12) Rabbit Monoclonal antibody M00820. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
For m00820 (abways CY6908), could you ask abways to describe the 4 lanes for the WB image? The customer is requesting more information. https://www.bosterbio.com/anti-apg12-atg12-rabbit-monoclonal-antibody-m00820-boster.html?options=cart
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
Abways said the current WB image was wrong and send us a new image. We are going to update our product page.
Boster Scientific Support
Answered: 2017-05-26
Question
Do you have a BSA free version of anti-Apg12 (Atg12) Rabbit Monoclonal antibody M00820 available?
D. Parker
Verified customer
Asked: 2015-05-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Apg12 (Atg12) Rabbit Monoclonal antibody M00820 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain ovary using anti-Apg12 (Atg12) Rabbit Monoclonal antibody M00820. Let me know if you need anything else.
C. Taylor
Verified customer
Asked: 2014-01-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-08
